热带臭虫转录组分析  

Transcriptome analysis of Cimex hemipterus(Hemiptera:Cimicidae)

作　　者：李婷 廖嵩 徐业 王常禄 王建国[1] LI Ting;LIAO Song;XU Ye;WANG Chang-lu;WANG Jian-guo(L aboratory of Invasion Biology,School of Agricultural Sciences,Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China;Department of Entomology,Rutgers,The State University of New Jersey,New Brunswick,NJ 08901,USA)

机构地区：[1]江西农业大学入侵生物实验室,江西南昌330045 [2]Department of Entomology,Rutgers,The State University of New Jersey,New Brunswick,NJ 08901,USA

出　　处：《中国媒介生物学及控制杂志》2022年第1期54-61,共8页Chinese Journal of Vector Biology and Control

基　　金：江西省2018年“双千人才”计划(jxsq2018102116);江西省国家外国专家项目(G20200222010,G2021022002)。

摘　　要：目的获得热带臭虫转录组数据,为后续基因功能研究奠定基础。方法基于Illumina NovaSeq 6000高通量测序平台,对热带臭虫混合样本进行测序,采用Trinity软件组装有效测序数据获得unigenes。将unigenes分别与非冗余蛋白数据库(NR)、基因本体论(GO)、京都基因和基因组百科全书(KEGG)、直系同源蛋白分组比对数据库(eggNOG)和注释蛋白数据库(Swiss-Prot)进行比对和功能注释,利用MISA软件对热带臭虫的unigenes进行简单重复序列位点分析,使用Samtools和GATK软件检测热带臭虫单核苷酸多态性(SNP)。结果转录组测序数据质控后获得5.49 Gb高质量数据,组装后获得21619个unigenes,总长度为29322540 bp,平均长度为1356 bp。将unigenes序列与5个公共数据库进行比对和功能注释,得到45362个有功能注释的unigenes,预测发现10906条编码区序列,检测到7754个简单重复序列(SSR)位点信息和33144个SNP位点信息。结论报道并构建了热带臭虫转录组数据库,并对21619个unigenes进行组装和功能注释分析,该数据的获得可为后期热带臭虫的功能基因研究、SSR和SNP分子标记开发、遗传多样性分析以及遗传图谱构建等奠定基础。Objective To obtain the transcriptome data of Cimex hemipterus and to provide a basis for subsequent functional genomic studies.Methods The mixed sample of C.hemipterus was sequenced on the Illumina NovaSeq6000 high-throughput sequencing platform.Unigenes were obtained by assembling the sequencing data using the Trinity software for alignment and functional annotation against the databases of non-redundant protein sequence(NR),Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG),Evolutionary Genealogy of Genes:Non-supervised Orthologous Groups(eggNOG),and Swiss-Prot.The unigenes were analyzed for simple sequence repeat(SSR)loci with MISA and single nucleotide polymorphism(SNP)loci with Samtools and GATK softwares.Results A total of 5.49 Gb high-quality data were obtained from quality-controlled raw sequencing data.A total of 21619 unigenes with a total length of 29322540 bp and an average length of 1356 bp were obtained.Alignment and functional annotation of the unigenes sequences with five public databases resulted in 45362 functional unigenes,10906 coding sequences(CDSs),7754 SSR loci,and 33144 SNPs.Conclusion The transcriptome database of C.hemipterus was established and 21619 unigenes were assembled for functional annotation analysis,which lays a foundation for subsequent studies of C.hemipterus in terms of functional genes,molecular marker development of SSR and SNP,genetic diversity,and genetic maps.

关 键 词：热带臭虫 转录组 生物信息学 微卫星 单核苷酸多态性 

分 类 号：R384.9[医药卫生—医学寄生虫学]