利用分子标记辅助选择Pigm-1基因改良恢复系R20的稻瘟病抗性  

Improving Blast Resistance of R20 by Molecular Marker-Assisted Selection of Pigm-1 Gene

作　　者：何旎清 杨德卫[1] 郑向华[1] 黄凤凰[1] 程朝平[1] 叶宁[1] HE Niqing;YANG Dewei;ZHENG Xianghua;HUANG Fenghuang;CHENG Chaoping;YE Ning(Institute of Rice,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350018)

机构地区：[1]福建省农业科学院水稻研究所,福建福州350018

出　　处：《核农学报》2022年第2期245-250,共6页Journal of Nuclear Agricultural Sciences

基　　金：福建省属公益项目(2020R1023003);福建省科技重大专项(2020NZ08016);福建省属公益项目(2018R1021-3);福建省农业高质量发展超越“5511”协同创新工程(XTCXGC2021001)。

摘　　要：水稻稻瘟病是水稻的重要病害之一,给我国甚至全世界的水稻安全生产带来了巨大隐患。选育优质抗稻瘟病恢复系,进而培育稻瘟病水稻杂交组合,是选育抗稻温病品种的有效途径之一。本研究以双抗77009为抗性基因Pigm-1的供体亲本,以感病恢复系R20为受体亲本,通过杂交和连续回交方法,并利用分子标记辅助选择技术,定向改良优质水稻恢复系R20的稻瘟病抗性,得到4个R20的抗病改良系。室内接菌和田间自然诱发鉴定结果表明,R20-1、R20-2、R20-3和R20-4改良系均表现为抗稻瘟病;遗传背景恢复分析显示,这4个改良系基本恢复受体亲本的背景;农艺性状分析表明,R20-1、R20-2、R20-3与受体亲本R20的株高、穗长、有效穗、每穗颖花数、结实率、粒长、粒宽和千粒重等性状相比,没有显著差异,而R20-4的粒长和千粒重与R20相比显著增加。本研究利用分子标记辅助技术改良了恢复系R20稻瘟病抗性,而且改良系R20-4在抗病的同时产量得到了提高,为抗稻瘟病基因Pigm-1的分子育种研究提供了参考依据。Rice blast is one of the most important rice diseases,which brings great hidden danger to the safety and production of rice in China and even the whole world.Developing functional markers of broad-spectrum R gene combined with molecular marker assisted selection is one of the most effective methods to improve blast resistance of rice.In this study,Shuangkang 77009 was used as the donor parent of the resistance gene Pigm-1,and the susceptible restorer line R20 was used as the recipient parent.Through hybridization,backcross,and molecular marker assisted selection technology,four improved lines of R20 with enhanced blast resistance were obtained.Indoor inoculation and field naturally induced identification showed that the improved lines R20-1,R20-2,R20-3 and R20-4 were resistant to rice blast.Background restoration analysis showed that the four improved lines basically restored the background of the recipient parent.Compared with R20,the agronomic traits of R20-1,R20-2 and R20-3 showed no significant differences in plant height,panicle length,effective panicle,spikelet number per panicle,seed setting rate,grain length,grain width and thousand seeds weight.However,the grain length and thousand seeds weight of R20-4 were significantly increased compared to those of R20.This study indicated that the utilization of molecular marker assisted selection technology not only improved the resistance of the restorer R20,but also increased the yield in R20-4.This study provides some value and reference for molecular breeding of rice blast resistance gene Pigm-1.

关 键 词：水稻稻瘟病 Pigm-1 分子标记辅助育种 背景恢复率 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]