使用活化诱导标记法评价HIV-1感染者特异性CD4^(+)T细胞免疫应答的研究  

Activation-induced markers for evaluation of specific CD4^(+)T cell immune response in HIV-1-infected patients

作　　者：王倩莹 王甜甜 王硕[1] 彭虹[1] 洪坤学[1] 邵一鸣 李丹[1] Wang Qianying;Wang Tiantian;Wang Shuo;Peng Hong;Hong Kunxue;Shao Yiming;Li Dan(State Key Laboratory of Infectious Disease Prevention and Control,National Center for AIDS/STD Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Department of Pediatrics,Tai′an Maternal and Child Health Hospital,Tai′an 271000,China)

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制国家重点实验室,北京102206 [2]泰安市妇幼保健院儿内科,泰安271000

出　　处：《中华微生物学和免疫学杂志》2022年第1期23-30,共8页Chinese Journal of Microbiology and Immunology

基　　金：传染病预防控制国家重点实验室优青课题(2019SKLID402);十三五科技重大专项(2018ZX10731-101)。

摘　　要：目的:建立一种检测HIV-1特异性CD4^(+)T细胞亚群功能的活化诱导标记法(activation-induced markers,AIM),从而更有效地评价HIV-1抗原特异性CD4^(+)T细胞免疫应答水平。方法:选取12例未经抗病毒治疗的HIV-1慢性感染者及6例未感染HIV-1的健康人,分别以基于多色流式细胞术的AIM法和细胞内因子染色法(intracellular cytokine staining,ICS)检测抗原特异性T淋巴细胞功能,并探讨两种方法用于评价HIV-1感染者抗原特异性T细胞免疫应答的能力。结果:AIM法检测HIV-1慢性感染者中HIV-1抗原特异性PD-1^(+)CD25^(+)CD4^(+)T、CD69^(+)CD200^(+)CD4^(+)T、CD69^(+)ICOS^(+)CD4^(+)T细胞阳性的比例为11/12、8/12和7/12,检测CD69^(+)ICOS^(+)CD8^(+)T、CD137^(+)CD69^(+)CD8^(+)T、PD-1^(+)CD25^(+)CD8^(+)、OX40^(+)PD-1^(+)CD8^(+)T细胞阳性的比例为8/12、8/12、7/12、7/12。ICS法检测HIV-1抗原特异性IL-2^(+)CD4^(+)T、IFN-γ^(+)CD4^(+)T、TNF-α^(+)CD4^(+)T细胞阳性的占比为2/12、2/12、0;IFN-γ^(+)CD8^(+)T、TNF-α^(+)CD8^(+)T、IL-2^(+)CD8^(+)T细胞阳性的占比为12/12、10/12、5/12。结论:AIM法在评价CD4^(+)T细胞功能方面更为敏感,可作为ICS法的补充,两种方法联合使用可更全面地评估抗原特异性T淋巴细胞反应。Objective To establish a method using activation-induced markers(AIM)to detect the function of HIV-1-specific CD4^(+)T cell subsets for evaluating the immune response of HIV-1-specific CD4^(+)T cells more effectively.Methods Twelve chronically HIV-1-infected patients without antiviral therapy and six healthy people without HIV-1 infection were enrolled in this study.The function of HIV-1-specific T lymphocytes was detected by AIM and ICS based on polychromatic flow cytometry.The performance of the two methods in assessing HIV-1-specific CD4^(+)T cell immune response in HIV-1-infected patients was evaluated.Results The positive rates of HIV-1-specific PD-1^(+)CD25^(+)CD4^(+)T,CD69^(+)CD200^(+)CD4^(+)T,CD69^(+)ICOS^(+)CD4^(+)T,CD69^(+)ICOS^(+)CD8^(+)T、CD137^(+)CD69^(+)CD8^(+)T、PD-1^(+)CD25^(+)CD8^(+)T and OX40^(+)PD-1^(+)CD8^(+)T cells in all of the HIV-1 patients were 11/12,8/12,7/12,8/12,8/12,7/12 and 7/12 using AIM method.ICS results showed that the positive rates of HIV-1-specific IL-2^(+)CD4^(+)T,IFN-γ^(+)CD4^(+)T,TNF-α^(+)CD4^(+)T,IFN-γ^(+)CD8^(+)T,TNF-α^(+)CD8^(+)T and IL-2^(+)CD8^(+)T cells were 2/12,2/12,0,12/12,10/12 and 5/12,respectively.Conclusions AIM method was more sensitive in antigen-specific CD4^(+)T cell detection,and could be used as a complementary method to ICS in assessing antigen-specific T cell response.

关 键 词：流式细胞术 活化诱导标记 胞内细胞因子染色 CD4^(+)T淋巴细胞 

分 类 号：R512.91[医药卫生—内科学]