大丽轮枝菌甘油-3-磷酸脱氢酶的功能分析  

Functional analysis of the glycerol-3-phosphate dehydrogenase gene in Verticillium dahliae

作　　者：刘涛 秦君 王春生 商文静[1] 胡小平[1] LIU Tao;QIN Jun;WANG Chunsheng;SHANG Wenjing;HU Xiaoping(State Key Laboratory of Crop Stress Biology in Arid Areas,College of Plant Protection,Northwest Agriculture and Forestry University,Yangling 712100,Shaanxi,China;College of Agronomy,Xinyang Agriculture and Forestry University,Xinyang 464006,Henan,China)

机构地区：[1]西北农林科技大学植物保护学院旱区作物逆境生物学国家重点实验室,陕西杨凌712100 [2]信阳农林学院,河南信阳464006

出　　处：《菌物学报》2022年第2期248-259,共12页Mycosystema

基　　金：科技部国际合作重点专项(2018YFE0112500);国家自然科学基金(31371888);陕西省自然科学基础研究计划资助项目(2021JQ-146);中央高校基本科研业务费专项资金(2452021136)。

摘　　要：大丽轮枝菌可侵染棉花、马铃薯、番茄等660余种寄主植物,引致黄萎病,造成严重的经济损失。为了深入了解大丽轮枝菌的致病机制,本研究在前期棉花提取物诱导大丽轮枝菌转录组分析的基础上,选择上调差异表达的线粒体甘油-3-磷酸脱氢酶基因VdGut2(VD592_6958_Chr4)和非差异表达的胞质甘油-3-磷酸脱氢酶基因VdGpd(VD592_10256_Chr2),进行了功能分析。结果表明,两个VdGut2敲除突变体菌株的产孢量较野生型菌株分别下降了32%和41%,病情指数分别下降了70%和51%,菌落生长速率也显著下降;VdGpd过表达菌株产孢量和病情指数较野生型菌株显著下降,但在甘油为唯一碳源的培养基上其菌落生长速率显著上升。因此,VdGut2促进了大丽轮枝菌分生孢子的形成、碳源的利用以及对寄主植物的致病过程,VdGpd抑制了大丽轮枝菌分生孢子的形成和对寄主植物的致病力,却促进了甘油的代谢。Verticillium dahliae can infect more than 660 species of host plants such as cotton,potato and tomato,causing Verticillium wilt and serious economic losses.In order to understand the pathogenic mechanism of V.dahliae,the up-regulated differentially expressed gene encoding mitochondrial glycerol-3-phosphate dehydrogenase VdGut2(VD592_6958_Chr4)and non-differentially expressed gene encoding cytosolic glycerol-3-phosphate dehydrogenase VdGpd(VD592_10256_Chr2)were selected for functional analysis based on the transcriptomic analysis induced by cotton extract.The results showed that the conidial production of the two VdGut2 knockout mutant strains decreased by 32%and 41%,respectively,the disease index decreased by 70%and 51%,and the colony growth rate also decreased significantly.On the other hand,the conidial production and disease index of the VdGpd overexpression strains significantly decreased,but the colony growth rate significantly increased on the medium with glycerol as the sole carbon source.Therefore,VdGut2 promotes the formation of V.dahliae conidia,the utilization of carbon source and the pathogenicity to host plant,while VdGpd inhibits the formation of V.dahliae conidia and the pathogenicity to host plant,but promotes the metabolism of glycerol.

关 键 词：甘油-3-磷酸脱氢酶 产孢量 碳源利用 致病性 

分 类 号：S432.44[农业科学—植物病理学]