过表达野生大豆S-腺苷甲硫氨酸合成酶基因GsSAMS提高水稻耐盐碱性  

Overexpression of A Glycine soja S-adenosylmethionine Synthetase GsSAMS in Rice Increases Salt-alkaline Tolerance

作　　者：才晓溪 沈阳 胡冰霜 王研 陈悦 孙明哲 贾博为 孙晓丽 CAI Xiaoxi;SHEN Yang;HU Bingshuang;WANG Yan;CHEN Yue;SUN Mingzhe;JIA Bowei;SUN Xiaoli(Crop Stress Molecular Biology Laboratory,College of Agriculture,Heilongjiang Bayi Agriculture University,Daqing,Heilongjiang 163319)

机构地区：[1]黑龙江八一农垦大学农学院作物逆境分子生物学实验室,黑龙江大庆163319

出　　处：《核农学报》2022年第1期50-56,共7页Journal of Nuclear Agricultural Sciences

基　　金：黑龙江八一农垦大学研究生创新科研项目(YJSCX2019-Y02、YJSCX2019-Y06);国家自然科学基金面上项目(31971826、31771692)。

摘　　要：S-腺苷甲硫氨酸合成酶(SAMS)是生化反应中合成甲基供体的关键酶,在植物逆境响应中具有重要的作用。为了鉴定野生大豆S-腺苷甲硫氨酸合成酶GsSAMS基因对水稻盐碱胁迫耐性的影响,本研究采用USER克隆技术构建植物过表达载体,以农杆菌介导法侵染水稻愈伤组织,经过PCR、半定量PCR(RT-PCR)等分子检测获得GsSAMS转基因水稻株系;通过对比野生型和转基因水稻盐碱胁迫处理后的表型、存活率及过氧化物酶(POD)、过氧化氢酶(CAT)活性等指标,发现GsSAMS转基因株系耐盐碱性显著优于野生型。通过实时荧光定量PCR(qRT-PCR)分析发现盐碱胁迫处理后,OsM6PR1、OsNAC5、OsPOX1基因在转基因株系中的表达显著高于野生型,说明GsSAMS可通过提高抗氧化物酶POD和CAT活性及相关基因的表达,增强转基因水稻盐碱胁迫耐受性。本研究获得的耐盐碱水稻新株系对盐碱地的开发利用具有重要意义。S-adenosyl-L-methionine synthetase(SAMS)is a key enzyme in the synthesis of methyl donors in biochemical reactions,and plays an important role in plant stress response.In order to identify the effect of wild soybean GsSAMS gene on rice salt-alkaline tolerance,an overexpression vector of the GsSAMS gene was generated based on the USER cloning technology,and GsSAMS transgenic rice lines were obtained by using the recombinant Agrobacterium infecting rice callus,and identified by PCR and RT-PCR assays.By comparing the phenotype,survival rates,POD and CAT activity of the wild type and GsSAMS transgenic lines under salt-alkaline stress,we found that the GsSAMS-OX lines displayed significantly higher salt-alkaline tolerance than the wild type.Through qRT-PCR analysis,the transcript levels of OsM6PR1,OsNAC5 and OsPOX1 were significantly higher in the transgenic lines than those in the wild type after salt-alkaline treatment.These results indicated that GsSAMS could enhance the salt-alkaline tolerance of transgenic rice by increasing the activity of antioxidant enzymes POD and CAT and enhancing the expressions of related genes.The new salt-alkaline rice lines obtained in this study are of great significance for the development and utilization of salt-alkaline land.

关 键 词：野生大豆 S-腺苷甲硫氨酸合成酶 GsSAMS 盐碱胁迫 转基因水稻 

分 类 号：S565.1[农业科学—作物学]