E-cadherin在牦牛不同繁殖周期输卵管不同部位中的表达与定位  

Expression and Localization of E-cadherin in Different Parts of the Oviduct at Different Reproduction Cycles of Yak

作　　者：杨珊珊 何翃闳 刘敏清 赵凌 王靖雷 余四九 蔡得琪 潘阳阳 YANG Shanshan;HE Honghong;LIU Minqing;ZHAO Ling;WANG Jinglei;YU Sijiu;CAI Deqi;PAN Yangyang(College of Veterinary Medicine/Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep&Goat,Gansu Agricultural University,Lanzhou,Gansu 730070;Agriculture and Rural Bureau of Jinchuan District of Jinchang City of Gansu Province,Jinchang,Gansu 737100)

机构地区：[1]甘肃农业大学动物医学院/甘肃省牛羊胚胎工程技术研究中心,甘肃兰州730070 [2]甘肃省金昌市金川区农业农村局,甘肃金昌737100

出　　处：《核农学报》2022年第1期57-65,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31972760);甘肃省教育厅产业支撑引导项目(2019C-03);甘肃农业大学伏羲青年英才基金(Gaufx-02Y10);甘肃农业大学博士科研启动基金(GSAU-RCZX201701)。

摘　　要：为研究上皮钙粘蛋白(E-cadherin)在不同繁殖周期牦牛输卵管不同部位中的表达差异,本研究采集卵泡期、黄体期和妊娠期无临床病理表现的母牦牛(3~6岁)输卵管伞部、壶腹部和峡部的样品,将不同繁殖周期输卵管分别按照伞部、壶腹部和峡部分为9组,采用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹法(western-blotting)检测E-cadherin基因和蛋白在不同时期牦牛输卵管不同部位中的表达情况,运用免疫组织化学法检测E-cadherin在不同时期牦牛输卵管不同部位中的定位。结果显示,在卵泡期,E-cadherin基因和蛋白在输卵管壶腹部的表达量最高,显著高于伞部和峡部;在黄体期,E-cadherin基因和蛋白在输卵管峡部的表达量最高,显著高于伞部和壶腹部;在妊娠期,E-cadherin基因在输卵管壶腹部的表达量最高,显著高于伞部和峡部,而E-cadherin蛋白在输卵管壶腹部的表达量最高,显著高于伞部。在输卵管伞部,E-cadherin基因在黄体期的表达量最高,显著高于卵泡期和妊娠期,而E-cadherin蛋白在卵泡期的表达量最高,显著高于黄体期和妊娠期;在输卵管壶腹部,E-cadherin基因和蛋白在妊娠期的表达量最高,显著高于卵泡期和黄体期;在输卵管峡部,E-cadherin基因和蛋白在黄体期的表达量最高,显著高于卵泡期和妊娠期。免疫组织化学结果显示,E-cadherin主要分布于输卵管伞部、壶腹部和峡部的纤毛细胞、分泌细胞、基细胞、肌层和浆液腺等部位。E-cadherin在牦牛繁殖周期输卵管不同部位中的均有表达,且其基因和蛋白的表达存在显著差异,说明E-cadherin可能参与了牦牛卵母细胞的生长和成熟、精子储存和获能、受精及早期胚胎发育等一系列生殖过程。本研究为进一步明确E-cadherin对牦牛繁殖性能的生物学作用提供了基础资料。The objective of this study was carried out to study the expression differences of E-cadherin in different parts of the oviduct at different reproduction cycles of yak,and to provide some important theoretical foundations for understanding the effect of E-cadherin on it′s reproductive performance.The samples of the tubal umbrella,ampulla,and isthmus of female yaks(3~6 years old)with no clinical pathological manifestations during follicular,luteal and pregnant phases were collected,the different reproduction cycles of yak are divided into 9 groups according to the umbrella,ampulla and isthmus.Real-time PCR(qRT-PCR)and western-blotting were used to detect the expression of E-cadherin gene and protein.The expression of E-cadherin protein was localized by immunohistochemistry.The results showed that during follicular phase,the expression of E-cadherin gene and protein in the ampulla of the oviduct was significantly higher than that in the umbrella and isthmus.During luteal phase,the expression of E-cadherin gene and protein in the isthmus of the oviduct was significantly higher than that in the umbrella and ampulla.During pregnant phase,the expression of E-cadherin gene in the ampulla of the oviduct was significantly higher than that in the umbrella and isthmus,and the expression of E-cadherin protein in the ampulla of the oviduct was significantly higher than that in the umbrella In the oviduct umbrella,the expression of E-cadherin gene in the luteal phase was significantly higher than that in the follicular phase and the pregnant phase,and the expression of E-cadherin protein in the follicular phase was significantly higher than that in the luteal phase and the pregnant phase.In the oviduct ampulla,the expression of E-cadherin gene and protein in the pregnant phase was significantly higher than that in the follicular phase and luteal phase.In the oviduct isthmus,the expression of E-cadherin gene and protein in the luteal phase was significantly higher than that in the follicular phase and pregnant phase.The resul

关 键 词：上皮钙粘蛋白 牦牛 输卵管 繁殖周期 表达 

分 类 号：S823.85[农业科学—畜牧学]