sRNA SaaS对沙门氏菌粘附响应规律和胞外代谢物的影响效应  

Effect of sRNA SaaS on Adhesion Response and Extracellular Metabolites of Salmonella

作　　者：何淑雯 胡海静 陆许秋 王浩东 王虎虎[1] 徐幸莲[1] HE Shuwen;HU Haijing;LU Xuqiu;WANG Haodong;WANG Huhu;XU Xinglian(National Center of Meat Quality and Safety Control/Jiangsu Collaborative Innovation Center of Meat Production and Processing,Quality and Safety Control,Nanjing Agricultural University,Nanjing,Jiangsu 210095)

机构地区：[1]南京农业大学国家肉品质量安全控制工程技术研究中心/江苏省肉类生产与加工质量安全控制协同创新中心,江苏南京210095

出　　处：《核农学报》2022年第1期121-128,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金面上项目(31872911)。

摘　　要：sRNA SaaS (Salmonella adhesion associated sRNA)是近期在肉品源肠炎沙门氏菌(S. Entertidis NCM 61)中筛选出的一种新型调控因子。本研究通过对比野生株与SaaS缺失突变株的粘附响应规律,分析二者胞外代谢物的差异以揭示sRNA SaaS的具体功能及可能的作用机制。结果显示,sRNA SaaS对肠炎沙门氏菌粘附能力的影响具有温度依赖性。在37℃下,SaaS能够抑制该菌在不锈钢、玻璃和聚丙烯表面形成生物菌膜,且在聚丙烯表面的抑制作用尤为明显。进一步通过广泛靶向代谢组学分析发现,sRNA SaaS可能分别从菌体细胞数量和胞外多聚物产量两方面抑制肠炎沙门氏菌生物菌膜的形成。本研究结果有助于完善生物菌膜的理论体系,为研发生物菌膜新型控制技术提供新思路。sRNA SaaS(Salmonella adhesion associated sRNA) is a new type of regulatory factor recently screened in meat-borne Salmonella Enteritidis(S. Entertidis NCM 61). This study compared the adhesion response of the wild and the SaaS mutant strains and analyzed the extracellular metabolites of the two strains to reveal the specific function and possible mechanism of sRNA SaaS. The results showed that the effect of sRNA SaaS on the adhesion of S. Enteritidis was temperature-dependent. At 37℃, SaaS could inhibit biofilm formation on stainless steel, glass and polypropylene surfaces, and the inhibitory effect was particularly obvious on polypropylene surface. Further analysis realized by widely-targeted metabolomics revealed that sRNA SaaS might inhibit the biofilm formation of S. Enteritidis in terms of the number of bacterial cells and the production of extracellular polymer substances. The results of this study may help to improve the theoretical system of biofilm and provide novel insight for the development of new control technologies for biofilm.

关 键 词：沙门氏菌 SRNA SAAS 粘附 广泛靶向代谢组学 

分 类 号：TS201.3[轻工技术与工程—食品科学]