淫羊藿苷对大鼠股骨头坏死微血管内皮细胞的影响  

Effect of icariin on bone microvascular endothelial cells in femoral head necrosis models of rats

作　　者：岳聚安 高贺 张启栋[2] 余华晨 刘沛 文鹏飞 程立明[2] 郭万首[2] YUE Ju-an;GAO He;ZHANG Qi-dong;YU Hua-chen;LIU Pei;WEN Peng-fei;CHENG Li-ming;GUO Wan-shou(Aviation General Hospital,Beijing 100012,China;Department of Joint Surgery,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]航空总医院,北京1000121 [2]中日友好医院关节外科,北京100029

出　　处：《中国矫形外科杂志》2022年第3期248-253,共6页Orthopedic Journal of China

基　　金：国家自然科学基金面上项目(编号:81673776);国家自然科学基金青年基金项目(编号:81703896)。

摘　　要：[目的]进一步验证淫羊藿苷(icariin,ICA)是否可有效的调控激素诱导的股骨头BMECs中miRNA-23b表达失衡、保护BMECs功能、预防激素性ONFH的发生.[方法]90只雌性SD大鼠随机三组,每组30只动物.模型组给予脂多糖大剂量甲强龙建立股骨头坏死模型,并给予生理盐水灌胃.ICA组同模型组给药,并给予ICA(每天60 mg/kg)灌胃,连续灌注4周.空白组仅给予等量生理盐水.4周处理动物,取股骨头行组织学检查,另外,自股骨头分离股骨头微血管内皮细胞(bone microvascular endothelial cells,BMECs)进行鉴定、细胞凋亡检测和miRNA-23b表达的生物信息学分析.[结果]组织形态计量方面,模型组股骨头骨小梁的宽度和骨小梁面积明显低于空白组与ICA组(P<0.05),而空白组与ICA组之间无明显差异(P>0.05).模型组的空骨陷窝率明显高于ICA组和空白组(P<0.05).细胞凋亡方面,BMECs活细胞率由高至低依次为:空白组> ICA组>模型组(P<0.05),其中,模型组显著低于空白组和ICA组(P<0.05),而空白组和ICA组差异无统计学意义(P>0.05).早期凋亡细胞率由高至低依次为:模型组>ICA组>空白组(P<0.05),其中,模型组显著高于空白组(P<0.05),而空白组与ICA组的差异无统计学意义(P>0.05).生物信息分析表明:与空白组相比,模型组miRNA-23b表达下调(P<0.05),ICA组与空白组差异无统计学意义(P>0.05).[结论]ICA在此动物模型可有效预防激素性ONFH发生,同时可保护BMECs,BMECs中的miRNA-23b可能是ICA预防激素性ONFH的作用靶点.[Objective]To further verify whether icariin(ICA)regulate the expression imbalance of mirNA-23B in hormone-induced femoral head bone microvascular endothelial cells(BMECs),protect the function of BMECs,and prevent the occurrence of hormone-induced osteonecrosis of femoral head(ONFH).[Methods]Ninety female SD rats were randomly assigned to three groups with 30 animals in each group.The animals in the model group were given high dose of methylprednisolone with lipopolysaccharide to establish femoral head necrosis model,and were gavaged with normal saline.Those in the ICA group were given the drugs as the model group,and were simultaneously gavaged with ICA 60 mg/kg•d_1 intragastrically for 4 weeks.However,the rats in the blank group only received the same amount of normal saline.At 4 weeks,the femoral heads were harvested for histological examination.In addition,BMECs were isolated from the femoral heads for identification,detection of apoptosis and bioinformatics analysis of miRNA-23B expression.[Results]In term of histomor-phometry,the width and area of trabecular bone in the model group were significantly lower than those in blank group and ICA group(P<0.05),while which was not significantl different between blank group and ICA group(P>0.05).The ratio of empty bone lacunae in model group was significantly higher than that in ICA group and blank group(P<0.05).In terms of cell apoptosis,the active cell ratios of BMECs were ranked from high to low:the blank group>the ICA group>the model group(P<0.05),which in the model group was significantly lower than the blank group and ICA group(P<0.05),while there was no significant difference between blank group and ICA group(P>0.05).Conversely,the ratios of early apoptotic cells from high to low was:the model group>the ICA group>the blank group(PvO.05),in which the model group was significantly higher than the control group(P<0.05),whereas there was no a significant difference between blank group and ICA group(P>0.05).As results of bioinformation analysis,mirNA-23B express

关 键 词：淫羊藿苷 微血管内皮细胞 股骨头坏死 骨小梁 凋亡 

分 类 号：R318[医药卫生—生物医学工程]