玉蕊响应土壤盐胁迫与盐雾胁迫的生理差异机制  

Mechanism of physiological difference in response to substrate salinity and salt spray of Barringtonia racemosa seedlings

作　　者：卞阿娜 陆銮眉 BIAN Ana;LU Luanmei(Department of Biological Science and Biotechnology,Minnan Normal University,Key Laboratory of Characteristics Landscape Plants in Fujian and Taiwan,Zhangzhou,Fujian 363000,China)

机构地区：[1]闽南师范大学生物科学与技术学院,闽台特色园林植物福建省高校重点实验室,福建漳州363000

出　　处：《植物生理学报》2022年第2期415-424,共10页Plant Physiology Journal

基　　金：福建省科技重点项目(2017N0026);闽南师范大学创新团队项目(2018)。

摘　　要：在温室培养条件下,对盐生植物玉蕊(Barringtonia racemosa)的幼苗进行土壤盐胁迫和盐雾胁迫(0、10、20、30和40 g·L^(-1))处理,研究两种盐胁迫玉蕊的各项生理指标的变化,以探讨玉蕊响应土壤盐胁迫和盐雾胁迫的耐盐适应差异机制。结果表明:两种盐胁迫玉蕊幼苗生长都慢于对照组,低浓度盐胁迫对玉蕊幼苗的生长影响不显著,高浓度盐胁迫则抑制幼苗生长。土壤盐胁迫玉蕊幼苗的叶片坏死斑主要分布于叶片中心区和老叶,盐雾胁迫则主要分布于叶尖和(或)叶缘和新叶;土壤盐胁迫其叶片灰分含量显著高于对照组,低盐度盐雾胁迫则影响不大;两种盐胁迫其幼苗体内的Na^(+)和Cl^(-)含量都显著增加,且存在极显著强正相关(P=0.005),离子毒害主要是由Na^(+)和Cl^(-1)共同造成的,盐雾胁迫对其幼苗体内的离子毒害比土壤盐胁迫的大。两种盐胁迫玉蕊幼苗叶片K;/Na^(+)、Mg^(2+)/Na^(+)和Ca^(2+)/Na^(+)值均低于对照组,且随盐度递增呈先降后升趋势;两种盐胁迫玉蕊幼苗可溶性糖和脯氨酸含量都随盐度递增而递增,MDA含量、SOD和POD活性都随盐度递增呈先升后降趋势,相同盐度盐雾胁迫其活性都高于土壤盐胁迫。土壤盐胁迫玉蕊适生的临界盐浓度为20 g·L^(-1),盐雾胁迫玉蕊适生的临界盐浓度为30 g·L^(-1)。可溶性糖含量、脯氨酸含量、MDA含量、SOD活性和POD活性可作为玉蕊幼苗盐胁迫响应的生理指标。To understand the mechanism of salt damage on halophyte Barringtonia racemosa seedlings,the seedlings were subjected to a substrate salinity gradient and salt spray (0,10,20,30,40 g·L^(-1)) in the greenhouse,and the changes of some physiological indexes were studied,which may explore the mechanism of physiological difference in response to substrate salinity and salt spray of B.racemosa Seedlings.The results demonstrated that B.racemosa seedlings treated with substrate salinity and salt spray exhibited slower growth rates than the control group.Low concentration salt stress had no significant on the growth of B.racemosa seedlings,while high concentration salt stress inhibited the growth of B.racemosa seedlings.The leaf necrotic spots were mainly distributed in the center part of leaves and the old leaves under substrate salinity,and the leaf necrotic spots were mostly distributed at the tip and edge of leaves and the new leaves under salt spray.The accumulation of ash content increased under substrate salinity,but which did not increase significantly under low salinity salt spray.The contents of Na^(+) and Cl^(-) in seedlings increased significantly under the two methods of salt stress,and there was a very significant positive correlation (P=0.005).The ion toxicity was mainly caused by Na^(+) and Cl^(-) and the ion toxicity in seedlings under salt spray was greater than that under substrate salinitys.The ratios of K^(+)/Na^(+),Mg^(2+)/Na ^(2+) and Ca^(2+)/Na^(+) in B.racemosa seedlings were lower than those in the control group under the two methods of salt stress,which decreased first and then increased with the increase of salinity.The contents of soluble sugar and proline in leaves of Vitex rotundifolia increased gradually,while the activities of SOD and POD,MDAcontent increased first and then decreased with the increase of salinity.Under the same salinity,the activities of SOD and POD,MDA content of salt spray were higher than those of substrate salinitys.The critical salt concentration of B.racemosa seeding

关 键 词：玉蕊 土壤盐胁迫 盐雾胁迫 离子分布 生理特性 

分 类 号：Q945.78[生物学—植物学]