Natural alleles of a uridine 5'-diphospho-glucosyltransferase gene responsible for differential endosperm development between upland rice and paddy rice  

作　　者：Zihao Wu Xiao Zhang Guimei Chang Jun Yang Jinpeng Wan Feijun Wang Dayun Tao Jiawu Zhou Lianguang Shang Peng Xu Diqiu Yu 

机构地区：[1]School of Life Sciences,University of Science and Technology of China,Hefei 230027,China [2]CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,the Chinese Academy of Sciences,Kunming 650223,China [3]Yunnan Key Laboratory for Rice Genetic Improvement,Food Crops Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650200,China [4]The Innovative Academy of Seed Design,Chinese Academy of Sciences,Mengla 666303,China [5]Shenzhen Branch,Guangdong Laboratory of Lingnan Modern Agricultural and Rural Affairs,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518000,China [6]Center of Economic Botany,Core Botanical Gardens,Chinese Academy of Sciences,Mergla 666303,China [7]State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan University,Kunming 650091,China

出　　处：《Journal of Integrative Plant Biology》2022年第1期135-148,共14页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.U1702231,31360330,31902110);The Science and Technology Projects of Yunnan Province,China(Grant No.202003AD150007);Strategic Leading Science and Technology Program of Chinese Academy of Sciences(Grant Nos.XDA24030301 and XDA24040308);Natural Science Foundation of Yunnan,China(Grant No.2018FA 023)。

摘　　要：Traditional upland rice generally exhibits insufficient grains resulting from abnormal endosperm development compared to paddy rice. However, the underlying molecular mechanism of this trait is poorly understood. Here,we cloned the uridine 5’-diphospho(UDP)-glucosyltransferase gene EDR1(Endosperm Development in Rice) responsible for differential endosperm development between upland rice and paddy rice by performing quantitative trait loci analysis and map-based cloning. EDR1 was highly expressed in developing seeds duringgrain filling. Natural variations in EDR1 significantly reduced the UDP-glucosyltransferase activity of EDR1 YZNcompared to EDR1 YD1,resulting in abnormal endosperm development in the near-isogenic line, accompanied by insufficient grains and changes in grain quality.By analyzing the distribution of the two alleles EDR1 YD1 and EDR1 YZNamong diverse paddy rice and upland rice varieties, we discovered that EDR1 was conserved in upland rice, but segregated in paddy rice. Further analyses of grain chalkiness in the alleles of EDR1 YD1 and EDR1 YZNvarieties indicated that rice varieties harboring EDR1 YZNand EDR1 YD1 preferentially showed high chalkiness, and low chalkiness,respectively. Taken together, these results suggest that the UDP-glucosyltransferase gene EDR1 is an important determinant controlling differential endosperm development between upland rice and paddy rice.

关 键 词：EDR1 endosperm development grain quality UDP-GLUCOSYLTRANSFERASE upland rice 

分 类 号：S511[农业科学—作物学]