A G-type lectin receptor-like kinase regulates the perception of oomycete apoplastic expansin-like proteins  

作　　者：Lei Pi Zhiyuan Yin Weiwei Duan Nan Wang Yifan Zhang Jinghao Wang Daolong Dou 

机构地区：[1]College of Plant Protection,China Agricultural University,Beijing 100193,China [2]College of Plant Protection,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Journal of Integrative Plant Biology》2022年第1期183-201,共19页植物学报（英文版）

基　　金：supported by the Fundamental Research Funds for the Central Universities(KYLH201703);the National Natural Science Foundation of China(32072507)。

摘　　要：Phytophthora capsici is one of the most harmful pathogens in agriculture, which threatens the safe production of multiple crops and causes serious economic losses worldwide. Here, we identified a P. capsici expansin-like protein, Pc EXLX1, by liquid chromatography-tandem mass spectrometry from Nicotiana benthamiana apoplastic fluid infected with P. capsici. Clustered regularly interspaced short palindromic repeats/crispr associated protein9(CRISPR/Cas9)-mediated Pc EXLX1 knockout mutants exhibited significantly enhanced virulence,while the overexpression of Pc EXLX1 impaired the virulence. Prokaryotically expressed Pc EXLX1 activated multiple plant immune responses, which were BRI1-associated kinase 1(BAK1)-and suppressor of BIR1-1(SOBIR1)-dependent. Furthermore, overexpression of Pc EXLX1 homologs in N. benthamiana could also increase plant resistance to P. capsici. A G-type lectin receptor-like kinase from N. benthamiana, expansin-regulating kinase 1(ERK1), was shown to regulate the perception of Pc EXLX1 and positively mediate the plant resistance to P. capsici. These results reveal that the expansin-like protein, Pc EXLX1, is a novel apoplastic effector with plant immunity-inducing activity of oomycetes, perception of which is regulated by the receptor-like kinase, ERK1.

关 键 词：apoplastic effector ELICITOR Nicotiana benthamiana OOMYCETE receptor-like kinase 

分 类 号：S432.4[农业科学—植物病理学]