RP-HPLC-ELSD同时测定滇女金丸中5个皂苷类成分的含量  

Simultaneous Determination of Five Saponins in Diannvjin Pills by RP-HPLC-ELSD

作　　者：仲侃 张紫佳[1] 饶正云 冯宝文 王朝军 ZHONG Kan;ZHANG Zi-jia;RAO Zheng-yun;FENG Bao-wen;WANG Zhao-jun(Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;HongWing Pharma(Kunming)Co.,Ltd.,Kunming 650033,China)

机构地区：[1]上海中医药大学中药研究所,上海201203 [2]红云制药(昆明)有限公司,云南昆明650033

出　　处：《中国现代中药》2022年第2期332-336,共5页Modern Chinese Medicine

基　　金：国家自然科学基金项目(81473322)。

摘　　要：目的:建立同时测定滇女金丸中的三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)、川续断皂苷Ⅵ、黄芪甲苷含量的反相高效液相-蒸发光散射检测法(RP-HPLC-ELSD)。方法:该药物的分析采用Agilent ZORBAX SB-C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为水-乙腈,梯度洗脱;流速为1 mL·min^(-1);柱温为25℃;漂移管温度为50℃;体积流量为1.6 L·min^(-1);增益5.0。结果:三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)、川续断皂苷Ⅵ、黄芪甲苷分别在0.874~10.920 (r=0.999 7)、1.264~10.110 (r=0.999 6)、0.627~10.030 (r=0.999 9)、0.653~13.572 (r=0.999 7)、1.018~10.180μg (r=0.9998)呈良好线性关系,平均回收率分别为102.07%(RSD为4.78%)、103.98%(RSD为4.61%)、99.39%(RSD为1.96%)、95.72%(RSD为3.76%)和101.64%(RSD为1.31%)。结论:该分析方法准确性、重复性及稳定性均良好,可用于滇女金丸的质量标准提升。Objective:To establisha reserved phase high performance liquid chromatogram-light scattering detector(RP-HPLC-ELSD) method for the simultaneous determination of notoginsenoside R_(1),ginsenoside Rg_(1),ginsenoside Rb_(1),asperosaponin Ⅵ,and astragaloside Ⅵ in Diannvjin Pills.Methods:The analysis was performed on an Agilent ZORBAX SB-C_(18)column (250 mm×4.6 mm,5μm) with gradient elution using water-acetonitrile as the mobile phase at the flow rate of1.0 mL·min^(-1),the column temperature of 25℃,the tube temperature of ELSD detector of 50℃,and a gas flow of 1.6 L·min^(-1)at gain in 5.0.Results:The calibration curves of notoginsenoside R_(1),ginsenoside Rg_(1),ginsenoside Rb_(1),asperosaponin Ⅵ,and astragalosideⅥwere in good linearity over the ranges of 0.874-10.920 (r=0.999 7),1.264-10.110 (r=0.999 6),0.627-10.030 (r=0.999 9),0.653-13.572 (r=0.999 7),and 1.018-10.180μg (r=0.999 8),with average recoveries of 102.07%(RSD of 4.78%),103.98%(RSD of 4.61%),99.39%(RSD of 1.96%),95.72%(RSD of 3.76%),and 101.64%(RSD of1.31%),respectively.Conclusion:This method is accurate,reproducible,and stable,and can be used for the quality control of Diannvjin Pills.

关 键 词：滇女金丸 含量测定 标准提升 

分 类 号：R286[医药卫生—中药学]