国兰叶色突变体根状茎差异表达基因分析  

Analysis of Differential Expression Genes in Rhizome of Leaf Color Mutant of Chinese Orchid

作　　者：蒋彧[1] 何俊蓉[1] JIANG Yu;HE Junrong(Institute of Horticulture,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610000)

机构地区：[1]四川省农业科学院园艺研究所,四川成都610000

出　　处：《核农学报》2022年第3期497-508,共12页Journal of Nuclear Agricultural Sciences

基　　金：四川省重点研发项目(2021YFN0118);四川省育种攻关项目(2021YFYZ0006);现代农业产业技术体系专项资金资助(CARS-23-G58);现代农业学科建设推进工程(2021XKJS047)。

摘　　要：兰花叶色对其观赏及商业价值具有非常重要的意义。国兰春剑隆昌素(Cymbidium longibracteaturn Longchangsu)及其叶色突变体叶艺隆昌素是研究兰花叶色形成机制的重要材料。本研究采用Illumina Hiseq2500对这2种材料的根状茎进行denovo转录组测序,以鉴定其与叶艺形成相关的差异表达基因(DEGs)。差异表达基因分析结果发现色素合成、叶绿体发育等相关的基因在隆昌素与叶艺隆昌素根状茎中的表达存在显著差异。本研究获得的序列数据极大地丰富了国兰可利用的基因资源,为进一步明确国兰叶色形成的分子机制提供了科学参考。The color of orchid leaf is very important for its ornamental and commercial value.The Chinese orchid Longchangsu and its leaf color mutant Yeyi Longchangsu are important materials to study the formation mechanism of the leaf color of orchid.Next-generation sequencing on Illumina Hiseq2500 platform was used to identify differentially expressed genes(DEGs)in the rhizomes of Longchangsu and Yeyi Longchangsu.The expressions of genes related to pigment synthesis and chloroplast development were significantly different between Longchangsu and Yeyi Longchangsu rhizomes.The sequence data obtained in this study greatly enriched the available gene resources of Chinese orchid and provided scientific reference for further clarifying the molecular mechanism of leaf color formation of Chinese orchid.

关 键 词：国兰 叶色 转录组 

分 类 号：S682.31[农业科学—观赏园艺]