血流介导组织荧光光谱测量系统及仿体验证  

Flow Mediated Tissue Fluorescence Measurement System and Phantom Verification

作　　者：张洋 何腾超 钟卫帅 董美丽 倪敬书 刘勇[1] 王贻坤 张元志 Zhang Yang;He Tengchao;Zhong Weishuai;Dong Meili;Ni Jingshu;Liu Yong;Wang Yikun;Zhang Yuanzhi(Anhui Institute of Optics and Fine Mechanics,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Anhui Provincial Engineering Technology Research Center for Biomedical Optical Instrument,Anhui Provincial Engineering Laboratory for Medical Optical Diagnosis&Treatment Technology and Instrument,Hefei,Anhui 230031;Science Island Branch,Graduate School of University of Science and Technology of China,Hefei,Anhui 230026,China;Wan Jiang New Industry Technology Development Center,Tongling,Anhui 244000,China)

机构地区：[1]中国科学院合肥物质科学研究院安徽光学精密机械研究所,安徽省生物医学光学仪器工程技术研究中心,安徽省医用光学诊疗技术与装备工程实验室,安徽合肥230031 [2]中国科学技术大学研究生院科学岛分院,安徽合肥230026 [3]皖江新兴产业技术发展中心,安徽铜陵244000

出　　处：《中国激光》2021年第24期141-150,共10页Chinese Journal of Lasers

基　　金：安徽省重点研究与开发计划(202004a07020016);安徽省自然科学基金(1908085QH365);中国科学院合肥物质科学研究院院长基金(YZJJZX202009);中国科学院科技服务网络计划(KFJ-STS-QYZD-184)。

摘　　要：基于血流介导组织还原型辅酶Ⅰ(NADH)荧光光谱检测技术的微循环功能评估对心血管病的早期预警具有重要价值,然而血流介导过程中氧饱和度的变化会干扰荧光信号的测量。本课题组搭建了集成有组织漫反射率测量模块的血流介导组织荧光光谱测量系统,并引入生理参数提取和荧光光谱复原算法,以排除干扰。不同光学参数的组织仿体试验结果表明,复原算法可以有效降低吸收、散射的影响,复原后的NADH的荧光强度与其浓度呈线性相关(拟合优度R^(2)=0.99);血液血氧仿体试验结果显示,组织氧饱和度的变化会干扰荧光的检测,经过校正的NADH的固有荧光光谱与氧饱和度的变化无关。4位受试者的在体试验结果显示,经过光谱复原后,荧光闭塞响应值和恢复响应值的平均值分别下降了22.8%和22.1%,这可以解释为氧饱和度的变化对实测NADH荧光的干扰。本研究结果预期能够有效提升血流介导组织荧光光谱测量系统的检测准确性。Objective The technique called flow-mediated NADH fluorescence measurement is used to reflect tissue microcirculation function,which has important value for early screening of cardiovascular disease.However,due to the obvious difference in the extinction coefficients of oxyhemoglobin and deoxyhemoglobin in the visible and nearinfrared bands,the tissue absorption coefficient will change constantly during the blood flow-mediated process.The excitation light and emission fluorescence of fluorescent molecules are affected by the tissue absorption coefficient.The current flow-mediated tissue fluorescence detection technology only measures the change in total tissue fluorescence intensity during brachial artery occlusion and release and does not account for the interference of tissue absorption coefficient changes caused by changes in oxygen saturation on NADH fluorescence measurement.Therefore,this problem may directly lead to blood flow-mediated tissue fluorescence technology failing to achieve an accurate measurement,thus limiting the clinical application of the technology.Methods First,we created the flow-mediated tissue fluorescence measurement system.The tissue absorption coefficient was calculated using a combination of tissue fluorescence and diffuse reflectance measurements.To improve the detection system’s accuracy,a steady-state tissue intrinsic fluorescence recovery method was used to correct the interference of absorption coefficient changes during the process of brachial artery occlusion and release on NADH fluorescence measurement.Second,we carried out the validation experiments of biological tissue solid phantom with different optical parameters and blood oxygen phantom simulating the physiological process of brachial artery occlusion and release.Furthermore,we validated the flow-mediated tissue fluorescence measurement system’s accuracy by comparing the corrected and uncorrected changes in blood flow-mediated tissue fluorescence in normal subjects.Results and Discussions The results of tissue solid

关 键 词：医用光学 组织荧光光谱 光谱复原 还原型辅酶Ⅰ 组织仿体 

分 类 号：R318.51[医药卫生—生物医学工程]