高效液相色谱法同时测定麻黄-桂枝药对中7种成分含量  

Simultaneous Determination of Seven Components in Ephedrae Herba-Cinnamomi Ramulus Herb Pairs by HPLC

作　　者：王东海 汤建华[1] 杨晓媛[1] 于小纬 WANG Donghai;TANG Jianhua;YANG Xiaoyuan;YU Xiaowei(The First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei,China 075000)

机构地区：[1]河北北方学院附属第一医院,河北张家口075000

出　　处：《中国药业》2022年第5期89-93,共5页China Pharmaceuticals

摘　　要：目的建立同时测定麻黄-桂枝药对中盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱、桂皮酸、桂皮醇、桂皮醛、香豆素7种成分含量的高效液相色谱法。方法色谱柱为Agilent Zorbax Eclipse Plus C18柱(250 mm×4.6 mm,5µm),流动相为乙腈-含0.1%NH4Cl的0.05%磷酸水溶液(梯度洗脱),流速为1.0 mL/min,检测波长为210 nm(盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱)、250 nm(桂皮酸、桂皮醇、桂皮醛、香豆素),柱温为30℃,进样量为10µL。结果盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱、香豆素、桂皮醇、桂皮醛和桂皮酸的质量浓度分别在45.400~567.500,18.050~225.600,4.584~57.300,1.648~20.600,2.836~35.440,11.250~140.700,1.195~14.940µg/mL(r=0.9993,0.9996,0.9995,0.9997,0.9994,0.9993,0.9997,n=6);平均加样回收率分别为98.81%,98.37%,98.14%,98.21%,98.39%,98.96%,98.19%,RSD分别为0.81%,0.77%,1.02%,1.02%,1.15%,0.72%,0.94%(n=6);精密度、重复性、稳定性试验的RSD均小于2.0%。结论该方法操作简便、结果准确、重复性好、精密度高,可用于测量麻黄桂枝-药对中7种成分的含量。Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous determination of seven components(ephedrine,pseudoephedrine,methylephedrine,cinnamic acid,cinnamic alcohol,cinnamaldehyde,coumarin)in Ephedrae Herba-Cinnamomi Ramulus herb pairs.Methods The chromatographic column was Agilent Zorbax Eclipse Plus C18 column(250 mm×4.6 mm,5µm),the mobile phase was acetonitrile-0.05%phosphoric acid solution containing 0.1%NH4Cl with gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was set at 210 nm for ephedrine,pseudoephedrine and methylephedrine,and 250 nm for cinnamic acid,cinnamic alcohol,cinnamaldehyde and coumarin,the column temperature was 30℃,and the injection volume was 10µL.Results The linear ranges of ephedrine hydrochloride,pseudoephedrine hydrochlorid,methylephedrine hydrochloride,coumarin,cinnamic alcohol,cinnamaldehyde and cinnamic acid were 45.400-567.500,18.050-225.600,4.584-57.300,1.648-20.600,2.836-35.440,11.250-140.700,1.195-14.940µg/mL(r=0.9993,0.9996,0.9995,0.9997,0.9994,0.9993,0.9997,n=6).The average recoveries of the above seven components were 98.81%,98.37%,98.14%,98.21%,98.39%,98.96%and 98.19%with RSDs of 0.81%,0.77%,1.02%,1.02%,1.15%,0.72%and 0.94%(n=6),respectively.The RSDs of precision,repeatability and stability tests were less than 2.0%.Conclusion The method is simple,accurate,reproducible and precise,which can be used for the determination of seven components in Ephedrae Herba-Cinnamomi Ramulus herb pairs.

关 键 词：高效液相色谱法 波长切换法 麻黄-桂枝药对 麻黄碱 伪麻黄碱 甲基麻黄碱 桂皮酸 桂皮醇 桂皮醛 香豆素 含量 

分 类 号：R932[医药卫生—生药学] R286.0[医药卫生—药学]