岩白菜素对D-GalN诱导大鼠急性肝损伤影响  

Effect of bergenin on D-galactosamine-induced acute liver injury in rats

作　　者：徐杰 高雅[1] 吉燕萍 连苑宇 张可锋[1] XU Jie;GAO Ya;JI Yan-ping(College of Pharmacy,Guilin Medical University,Guilin,Guangxi Zhuang Autonomous Region 541004,China)

机构地区：[1]桂林医学院药学院,广西桂林541004

出　　处：《中国公共卫生》2022年第1期70-74,共5页Chinese Journal of Public Health

基　　金：广西八桂学者专项(桂财教函[2017]143号)。

摘　　要：目的了解岩白菜素对D-GalN诱导急性肝损伤的保护作用及其作用机制。方法将60只SD大鼠随机分为对照组、模型组、水飞蓟素组(120 mg/kg)及岩白菜素低、中、高剂量组(20、40、80 mg/kg),每组10只,各剂量组大鼠每天同一时间按10 mL/kg灌胃给药,对照组和模型组大鼠灌胃相应体积蒸馏水,每天1次,连续7 d。末次给药2 h后,按4 mL/kg腹腔注射D-GalN(700 mg/kg)建立动物急性肝损伤模型。16 h后,收集血液,采集肝组织。生化法检测大鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、总抗氧化能力(T-AOC)、总胆红素(TBIL)、总超氧化物歧化酶(T-SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)活性或含量;酶联免疫吸附法(ELISA)检测大鼠肝组织中γ–干扰素(IFN-γ)、肿瘤坏死因子–α(TNF-α)、白介素–1β(IL-1β)和IL-6含量;蛋白免疫印记法(Western blot)分析肝组织p-JNK、JNK、c-Jun、p-AMPK和AMPK的蛋白表达情况;HE染色检查肝组织病变程度。结果与对照组比较,模型组大鼠血清中AST、ALT、TBIL活性和MDA含量明显升高(P <0.01);与模型组比较,各岩白菜素组大鼠血清中AST、ALT、TBIL活性和MDA含量明显降低(P <0.05),岩白菜素高、中剂量组大鼠血清中T-AOC、T-SOD和GSH-Px含量均升高(P <0.01);与模型组比较,岩白菜素高、中剂量组大鼠肝组织中TNF-α、IL-1β和IL-6含量下降、IFN-γ含量升高(P <0.01),p-JNK和c-Jun蛋白表达下调(P <0.05)、p-AMPK表达上调(P <0.01)。结论岩白菜素对D-GalN诱导的大鼠急性肝损伤具有保护作用,其作用机制可能与激活AMPK/JNK信号通路抑制氧化应激与炎症反应有关。Objective To study protective effect and mechanism of bergenin on D-galactosamine(D-GalN)-induced acute liver injury in rats. Methods Totally 60 Sprague-Dawley(SD) rats were randomly assigned into 6 groups(10 in each group): a control and a model group with saline, a silymarin group(120 mg/kg), and low, moderate and high bergenin groups(20, 40, 80 mg/kg). All the treatments were carried out by intragastric administration(10 mL/kg) once a day consecutively for7 days. Two hours after the last administration, D-GalN(700 mg/kg) was intraperitoneally administered for all the rats to establish acute liver injury model, excepted for the control rats. Sixteen hours after the D-GalN injections, all rats′ blood and liver tissue specimens were collected. Biochemical methods were used to detect serum content or activity of alanine aminotransferase(ALT), aspartate aminotransferase(AST), total antioxidant capacity(T-AOC), total bilirubin(TBIL), total superoxide dismutase(T-SOD), malondialdehyde(MDA), and glutathione peroxidase(GSH-Px);enzyme-linked immunosorbent assay(ELISA) was adopted to measure contents of interferon-γ(IFN-γ), tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β), and interleukin-6(IL-6) in liver tissues;and Western blot analysis was performed to determine phosphorylated c-Jun N-terminal kinase(p-JNK), JNK, c-Jun, phospharylated adenosine monophosphate-activated protein kinase(p-AMPK), and AMPK protein expressions in liver tissues, respectively. Pathological changes of liver tissues were observed with hematoxillin-eosin(HE) staining. Results Compared with those in the control rats, serum activity of AST,ALT, TBIL and MDA were significantly increased in the model rats(all P < 0.01). In contrast to those in the model rats,serum AST, ALT, TBIL, and MDA were significantly reduced in the rats of the three bergenin groups(all P < 0.05) and the activity of serum T-AOC, T-SOD, and GSH-Px were significantly increased in the rats of moderate and high bergenin groups(all P < 0.01);in addition, significantly dec

关 键 词：岩白菜素 急性肝损伤 氧化应激 腺苷酸活化蛋白激酶/应激活化蛋白激酶(AMPK/JNK)信号通路 

分 类 号：R99[医药卫生—毒理学]