二补助育改良方对高龄小鼠子宫内膜形态学及氧化应激的影响  

Effects of Erbu Zhuyu Gailiang Fang on endometrial morphology and oxidative stress in aged mice

作　　者：周雨玫 刘雁峰[1] 申萌萌 孙之迪 郑涵予 郑志博 ZHOU Yumei;LIU Yanfeng;SHEN Mengmeng;SUN Zhidi;ZHENG Hanyu;ZHENG Zhibo(Dongnhimen Hospital,Beijing University of Chinese Medicine,Bejing 100700,China;Third Affiliated Hospital of Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学第三附属医院

出　　处：《北京中医药大学学报》2022年第2期157-164,共8页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(No.82074480)。

摘　　要：目的探讨二补助育改良方对高龄小鼠子宫内膜形态学及氧化应激相关指标的影响。方法将8~9月龄妊娠小鼠据随机数字表分为高龄模型组、补佳乐组(0.3 mg/kg)、阿司匹林组(15 mg/kg)和二补助育改良方低、中、高(10.89、21.78、43.56 g/kg)剂量组,另选择6~8周龄妊娠小鼠为育龄空白组,每组8只。观察子宫大体形态、子宫内膜形态、子宫内膜胞饮突、胚胎着床情况,计算各组胞饮突评分、妊娠率、平均着床位点数,WST-1法测定血清与子宫内膜中超氧化物歧化酶(SOD)活力,比色法测定丙二醇(MDA)的含量。结果与育龄空白组比较,高龄模型组子宫呈黯棕红色,管腔内有瘀血,内膜变薄,血管、腺体数量减少,微绒毛稀疏,胞饮突评分极低(P<0.05),平均着床位点数减少(P<0.05),血清、子宫SOD活力降低(P<0.05),血清、子宫MDA含量升高(P<0.05)。与高龄模型组比较,各治疗组子宫颜色、管腔内淤血、内膜厚度、血管与腺体数量均有不同程度改善,改良方低、中、高剂量组和补佳乐组胞饮突评分提高(P<0.05),改良方低、中、高剂量组平均着床位点数及妊娠率提高(P<0.05),改良方高剂量组与阿司匹林组血清SOD活力升高(P<0.05),改良方低、中、高剂量组与补佳乐组子宫SOD活力升高(P<0.05),改良方低、中、高剂量组和补佳乐组血清MDA含量降低(P<0.05),改良方中、高剂量组子宫MDA含量降低(P<0.05)。结论二补助育改良方可丰富内膜微绒毛和胞饮突的表达,提高SOD活力,降低MDA含量,改善血清与子宫氧化应激水平,从而改善子宫内膜容受性,提高胚胎着床位点数和妊娠率。Objective To investigate the effect of Erbu Zhuyu Gailiang Fang(Modified Formula of Promoting Fertility with Two Tonics, EBZYGLF) on endometrial morphology and oxidative stress related indexes in aged mice. Methods Pregnant mice aged 8~9 months were randomly divided into 6 groups by using random number table(n=8 in each group): aged model group, low-dose(10.89 g/kg), mid-dose(21.78 g/kg) and high-dose(43.56 g/kg) EBZYGLF groups, progynova group(0.3 mg/kg) and aspirin group(15 mg/kg). In addition, 8 pregnant mice aged 6~8 weeks were selected as the childbearing blank group. The morphology of uterus, HE staining of endometrium, pinopodes and embryo implantation were observed. Pinopodes scores, pregnancy rate and average number of embryo implantation sites were calculated. Superoxide dismutase(SOD) in serum and endometrium was measured by using WST-1 method. The content of malondialdehyde(MDA) was measured with colorimetry. Results Compared with the control group, the color of uterus in the model group was dark brownish red, with intraluminal blood stasis, thinner endometrium, fewer blood vessels and glands, sparse microvilli, very low pinopodes score(P<0.05), decreased average number of implantation sites(P<0.05), decreased SOD activity in serum and uterus(P<0.05), and increased MDA content in serum and uterus(P<0.05) with satistical significance. Compared with the aged model group, the uterine color, intraluminal blood stasis, endometrial thickness, the number of blood vessels and glands in each treatment group were improved in varying degrees. Pinopodes scores in the low-, mid-and high-dose EBZYGLF groups and the progynova group were increased(P<0.05). The average number of implantation sites and pregnancy rate in the low-, mid-and high-dose EBZYGLF groups were increased(P<0.05), SOD activity in serum increased in high-dose group and aspirin group(P<0.05), SOD activity in uterus increased in low, medium and high-dose groups of EBZYGLF and progynova group(P<0.05), the content of MDA in serum decreased in low-, mid

关 键 词：二补助育改良方 高龄小鼠 形态学 子宫内膜容受性 氧化应激 

分 类 号：R285.5[医药卫生—中药学]