Confocal laser speckle autocorrelation imaging of dynamic flow in microvasculature  

作　　者：E Du Shuhao Shen Anqi Qiu Nanguang Chen 

机构地区：[1]Department of Biomedical Engineering,National University of Singapore,7 Engineering Drive 1,Singapore 117574,Singapore

出　　处：《Opto-Electronic Advances》2022年第2期22-32,共11页光电进展（英文）

基　　金：supports from Ministry of Education-Singapore(MOE2019-T2-2-094,R-397-000-327-114).

摘　　要：Laser speckle imaging has been widely used for in-vivo visualization of blood perfusion in biological tissues.However,existing laser speckle imaging techniques suffer from limited quantification accuracy and spatial resolution.Here we re-port a novel design and implementation of a powerful laser speckle imaging platform to solve the two critical limitations.The core technique of our platform is a combination of line scan confocal microscopy with laser speckle autocorrelation imaging,which is termed Line Scan Laser Speckle Autocorrelation Imaging(LS-LSAI).The technical advantages of LS-LSAI include high spatial resolution(~4.4μm)for visualizing and quantifying blood flow in microvessels,as well as video-rate imaging speed for tracing dynamic flow.

关 键 词：laser speckle AUTOCORRELATION CONFOCAL line scan FLOW 

分 类 号：TN249[电子电信—物理电子学] TP391.41[自动化与计算机技术—计算机应用技术] R318[自动化与计算机技术—计算机科学与技术]