高精细水凝胶微图案的快速制备及其对细胞行为的诱导  

Rapid preparation of high-precision hydrogel micropatterns and its induction of cell behavior

作　　者：张维彩 郑美玲[1] 董贤子[1] 刘洁[1] 金峰[1] Zhang Weicai;Zheng Meiling;Dong Xianzi;Liu Jie;Jin Feng(Laboratory of Organic NanoPhotonics and CAS Key Laboratory of Bio-Inspired Materials and Interfacial Science,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China;School of Future Technology,University of Chinese Academy of Sciences,Beijing 101407,China)

机构地区：[1]中国科学院理化技术研究所仿生智能界面科学中心有机纳米光子学实验室,北京100190 [2]中国科学院大学未来技术学院,北京101407

出　　处：《光电工程》2022年第2期62-72,共11页Opto-Electronic Engineering

基　　金：国家自然科学基金资助项目(61975213,61475164,51901234);中奥国际合作项目(GJHZ1720);中国科学院国际伙伴计划(GJHZ2021130)。

摘　　要：为探索不同尺寸的水凝胶微图案对细胞的诱导调控作用,本文采用飞秒激光无掩模投影光刻技术,将水凝胶前驱体溶液制备成所设计的图案,同时结合大面积拼接,获得了具有大面积、不同尺寸的多边形和多角星微结构。详细研究了微结构的最佳加工条件及其浸润性。带有微结构的基底与成纤维细胞共培养的实验结果表明,微结构的空间限位作用会改变细胞形貌,从而能够对细胞的生长行为进行有效地调控。尤其在小尺寸的多边形和多角星微结构上,细胞核会落入微结构的中心陷窝,细胞骨架则不断铺展分布,并逐渐与微结构形貌趋于一致。该研究证实了微结构图案单元尺寸对诱导细胞行为功能至关重要,将为利用生物相容性水凝胶微结构进行体外细胞研究提供新技术与新方法。In order to investigate the regulation effect of hydrogel micropatterns of different sizes on cell behaviors,large-area polygons and polygonal microstructures of different sizes were achieved by a femtosecond laser maskless optical projection lithography technique,in combination with a large-area splicing method.The optimum processing conditions and the wettability of the microstructure are studied in detail.The coculture experimental results of fibroblasts with patterned substrates showed that the spatial confinement in the microstructure can change the cell morphology and effectively regulate the cell growth behaviors.In particular,in the small polygonal and polygonal star microstructure,the nucleus tends to fall into the concave lacunae of the microstructure,and the spreading of cytoskeleton gradually tends to be consistent with the morphology of microstructures.This study indicates that the size of microstructural pattern units is very important for inducing cell behavior and function,which would provide a new technology and new method for cell research in vitro by using biocompatible hydrogel microstructures.

关 键 词：无掩模投影光刻 水凝胶 微图案 细胞行为 

分 类 号：TH741.5[机械工程—光学工程] TN249[机械工程—仪器科学与技术]