基于双峰谐振长周期光纤光栅的H9N2亚型禽流感病毒传感器  

Biosensor Based on Dual-Resonance Long-Period Fiber Gratings for Detection of H9N2 Subtype Avian Influenza Virus

作　　者：石胜辉[1] 聂青林 蒋上海 吴胜昔[2] 汤斌[1] 赵明富[1] Shi Shenghui;Nie Qinglin;Jiang Shanghai;Wu Shengxi;Tang bin;Zhao Mingfu(Chongqing Key Laboratory of Optical Fiber Sensor and Photoelectric Detection,Chongqing University of Technology,Chongqing 400054,China;Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology,Chongqing University of Technology,Chongqing 400054,China)

机构地区：[1]重庆理工大学光纤传感与光电检测重庆市重点实验室,重庆400054 [2]重庆理工大学药物化学与分子药理学重庆市重点实验室,重庆400054

出　　处：《光学学报》2022年第1期51-61,共11页Acta Optica Sinica

基　　金：国家自然科学基金(61875026,61805029);重庆市教育委员会科学技术研究计划青年项目(KJQN201801121);重庆市自然科学基金面上项目(cstc2019jcyj-msxmX0093);重庆理工大学研究生创新项目(ycx20192046)。

摘　　要：H9N2亚型禽流感病毒(AIV)虽为低致病性AIV,但严重危害养禽业的健康发展和公共卫生系统。快速有效的检测方法有利于病毒的早期诊断、预防及控制。提出一种高特异性、低检测极限(LOD)的纳米二氧化钛(nano-TiO_(2))粒子修饰双峰谐振长周期光纤光栅(DR-LPFG)的检测H9N2 AIV的光学生物传感器。利用改性nano-TiO_(2)粒子修饰DR-LPFG,再将抗H9N2单克隆抗体分子(anti-H9N2 MAbs)与TiO_(2)表面羧基以共价键结合,固定于光栅表面制得生物传感器。该传感器机理在于测量固定在DR-LPFG表面的anti-H9N2 MAbs与H9N2 AIV抗原的特异性结合引起光栅双峰谐振波长间距(Δλ)的变化。实验结果表明:在折射率为1.3320~1.3760时,nano-TiO_(2)修饰DR-LPFG的Δλ灵敏度为~1063.44 nm/RIU(RIU为折射率单位)。该生物传感器对H9N2 AIV的LOD为~2.7 ng/mL,相较采用Eudragit L100共聚物修饰DR-LPFG的生物传感器的LOD,提高了约96.1%,检测饱和浓度为50μg/mL,对H9N2 AIV的亲和系数为~3.57×10^(8)mol^(-1)·L,对H9N2 AIV具有高特异性,且能实现快速检测,在临床诊断、药物分析等生物医学领域有较大应用潜力。H9 N2 subtype avian influenza virus(AIV) is a low-pathogenicity AIV that seriously threatens the healthy development of the poultry industry and public health systems. By developing rapid, sensitive, and accurate methods suitable for field detection of H9 N2 AIV, we could efficiently control the viral infection and its spread over time. This study proposes a novel biosensor with high specificity and a low limit of detection(LOD) for H9 N2 AIV detection. The sensor employs a dual-resonance long-period fiber grating(DR-LPFG) modified with TiO_(2) nanoparticles(nano-TiO_(2)). Anti-H9 N2 monoclonal antibody molecules(anti-H9 N2 MAbs) are covalently bound to the TiO_(2) surface carboxyl groups on the surface of the DR-LPFG, thus preparing the biosensor. The biosensor measures the variation of the dual-resonance wavelength spacing(Δλ) caused by the specific interaction between the fixed anti-H9 N2 MAbs on the surface of DR-LPFG and H9 N2 AIV antigen. In experimental tests, the Δλ sensitivity of the nano-TiO_(2) coated DR-LPFG sensor is about 1063.44 nm/RIU(where RIU is the refractive index unit) in the refractive index range 1.3320--1.3760. The LOD of the proposed biosensor for H9 N2 AIV is ~2.7 ng/mL, 96.1% greater than that of a DR-LPFG-based biosensor modified with Eudragit L100 copolymer. The biosensor saturates at 50 μg/mL, and its affinity coefficient to H9 N2 AIV is ~3.57×10^(8)mol^(-1)·L. The biosensor also shows a high specificity and a rapid detection for H9 N2 AIV, affirming its high application potential in biomedical fields such as clinical diagnosis and drug analysis.

关 键 词：光纤光学 光纤生物传感器 长周期光纤光栅 双峰谐振 纳米二氧化钛粒子 H9N2亚型禽流感病毒 

分 类 号：TN253[电子电信—物理电子学]