一种关联生附片强心作用的质量快速评价方法的建立  

Establishment of a rapid method for quality assessment of cardiotonic effect of Shengfupian

作　　者：陈露梦 贺亚男[1] 刘惠 谭鹏 袁海峰 马鸿雁[1] 林俊芝 杨明 贾明艳 张定堃[1] CHEN Lu-meng;HE Ya-nan;LIU Hui;TAN Peng;YUAN Hai-feng;MA Hong-yan;LIN Jun-zhi;YANG Ming;JIAMing-yan;ZHANG Ding-kun(Pharmacy School,State Key Laboratory of Southwestern Chinese Medicine Resources,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;State Key Laboratory of Biological Evaluation of TCM Quality,National Administration of TCM,Academy of Chinese Medicine Sciences,Chengdu 610041,China;Sichuan Xingshengyuan Pharmaceutical Co.,Ltd.,Jiangyou 621700,China;TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China;State Key Laboratory of Innovation Drug and Efficient Energy-saving Pharmaceutical Equipment,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China)

机构地区：[1]成都中医药大学药学院,西南特色中药资源国家重点实验室,四川成都611137 [2]四川省中医药科学院,国家中医药管理局中药质量生物评价重点研究室,四川成都610041 [3]四川新盛源药业有限公司,四川江油621700 [4]成都中医药大学附属医院,代谢性疾病中医药调控四川省重点实验室,四川成都610072 [5]江西中医药大学,现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《药学学报》2022年第2期460-466,共7页Acta Pharmaceutica Sinica

基　　金：四川省中医药管理局科学技术研究专项(2021MS016);国家自然科学基金青年科学基金项目(82003907);四川省科技创新(苗子工程)培育项目(2021041);四川省重点研发项目(22ZDYF0740)。

摘　　要：本研究基于附子强心组分去甲乌药碱(higenamine,HI)、去甲猪毛菜碱(salsolinol,SA)、棍掌碱(coryneine,CO)共有的肾上腺素类似结构,构建了以邻二酚羟基基团与茜素红S-苯硼酸体系置换反应为核心的光化学比色传感器,用于快速鉴别生附片的强心作用。研究表明,传感器最优条件为:茜素红S(alizarin red S,ARS)和苯硼酸(phenylboric acid,PA)的摩尔比1∶3、反应温度0℃;供试品制备方法优选为:取生附片粉末2.5 g,加10倍量甲醇,300 W、40 kHz超声15 min;方法学研究表明该方法精密度、重复性、稳定性良好。通过显色测定得出14个样品的|△G|值(绿色G,|△G|=|G后-G前|);采用LC-MS/MS测定生附片中3种强心成分的含量,发现强心成分的总含量排序与|△G|总体上基本一致;随后进行相关性分析,相关系数R;高达0.87,证明了本方法的科学性与准确性。该研究填补了生附片优质性快速评价方法的空白,为生附片市场流通与临床应用优质优价提供了技术支撑。Based on the similar structure of adrenaline shared by higenamine(HI),salsolinol(SA)and coryneine(CO),a photochemical colorimetric sensor based on the displacement reaction of o-diphenol hydroxyl group and alizarin red S-phenylboric acid system was constructed to quickly distinguish and identify the cardiac strength of Shengfupian.The results show that the optimal condition of the sensor is:the molar ratio of alizarin red S(ARS)to phenylboric acid(PA)is 1∶3,reaction temperature is 0℃;The preparation method of the sample solution is optimized as follows:2.5 g of Shengfupian powder was taken,10 times the amount of methanol was added,and 300 W,40 k Hz ultrasound was carried out for 15 min;methodological studies showed that the method had good precision,repeatability and stability.The|△G|value(G is green,|△G|=|G after-G before|)of each sample was obtained by response values determination of 14 batches of Shengfupian.LC-MS/MS was used to determine the contents of three cardiac components in Shengfupian.It was found that the order of the total contents of cardiotonic components was basically consistent with|△G|.Then the correlation was analyzed,and the correlation coefficient R;was as high as 0.87,which proved the scientificity and accuracy of this method.This study fills the methodological gap of rapid evaluation of the quality of Shengfupian,and provides the key technical support for the high quality and good price of Shengfupian in the market circulation and clinical application.

关 键 词：附子 强心作用 快检方法 茜素红S 苯硼酸 质量评价 

分 类 号：R917[医药卫生—药物分析学]