枸杞属植物nrDNA ITS致同进化不完全  

Incomplete concerted evolution of nrDNA ITS in Lycium

作　　者：张娇 聂颖兰[1] 郭威[2] 钱丹[1] 黄璐琦[3] ZHANG Jiao;NIE Ying-lan;GUO Wei;QIAN Dan;HUANG Lu-qi(Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases,Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China;Shandong Provincial Maternal and Child Health Care Hospital,Shandong University,Jinan 250014,China;China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院医学实验中心,中医药防治重大疾病基础研究北京市重点实验室,北京100700 [2]山东大学附属山东省妇幼保健院,山东济南250014 [3]中国中医科学院,北京100700

出　　处：《药学学报》2022年第2期525-532,共8页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81803671);中央级公益性科研院所基本科研业务费专项资金(ZZ13-YQ-079);中国中医科学院科技创新工程(CI2021A04104)。

摘　　要：为探讨枸杞属(Lycium)nrDNA ITS序列致同进化不完全现象及假基因问题,本研究以cDNA ITS为参照,对枸杞属栽培品种的ITS进行测序。综合GenBank数据库获取的44条枸杞属ITS序列进行分析。考察碱基变异、GC含量、5.8S保守基序、二级结构最小自由能、进化模式及系统发育关系等对枸杞属的ITS多态性和假基因进行鉴定。结果表明,枸杞属植物内存在明显的ITS多态性,植物种间及个体内存在致同进化不完全现象,144条序列中共83条序列被鉴定为假基因序列。本研究首次对枸杞属ITS多态性和假基因进行了较为系统地检验,阐述了枸杞属ITS假基因特性,评估了假基因对枸杞属系统进化的影响。为ITS序列用于枸杞属种质资源研究和DNA条形码鉴别研究等提供了参考。In this study,nrDNA ITS sequences of Lycium cultivars were sequenced and used to test the existence of incomplete concerted evolution and pseudogenes.Together with 44 ITS sequences retrieved from GenBank,the pattern of base substitutions,GC content,5.8 S conserved motifs,the minimum free energy of secondary structures,nucleotide diversity and phylogenetic relationship of the samples were analyzed.While 83 of the 144 sequences were identified as pseudogenes,the results suggested a high degree of polymorphism and putative pseudogenes in Lycium,suggesting an incomplete concerted evolution of the ITS region.ITS polymorphism and pseudogene of Lycium were systematically tested for the first time.This research provides a references for ITS sequence to be used in the study of Lycium germplasm resources and DNA barcode identification.

关 键 词：枸杞属 nrDNA ITS 多态性 致同进化不完全 假基因 

分 类 号：R931[医药卫生—生药学]