青海刚察某牧场牦牛犊牛腹泻病原的分子鉴定分析  

Molecular Identification and Analysis of Pathogens Causing Yak Calf Diarrhea in a Farm in Gangcha of Qinghai Province

作　　者：张学勇[1] 简莹娜 朵红[1] 马怡隽 付永[1] 沈秀英[1] 宋永武 才让周在 郭志宏[1] ZHANG Xue-yong(Academy of Animal Science and Veterinary Medicine of Qinghai University,Xining 810016)

机构地区：[1]青海大学畜牧兽医科学院,西宁810016 [2]青海刚察县畜牧兽医站,刚察812300

出　　处：《青海畜牧兽医杂志》2022年第1期23-29,55,共8页Chinese Qinghai Journal of Animal and Veterinary Sciences

基　　金：家畜主要疫病诊断与防控技术研究示范(2017-NK-110);青海大学中青年科研基金项目(2017-QNY-1)。

摘　　要：青海省刚察县吉尔孟乡某牧场发生牦牛犊牛腹泻疫病,为快速诊断发病犊牛腹泻的致病病原,保证及时采取防控治疗措施,特采集牦牛犊牛腹泻粪便样本8份,利用显微镜镜检方法和分子生物学方法进行鉴定分析。结果显示:此牧场中引发犊牛腹泻的病原是隐孢子虫(Cryptosporidium),贾第鞭毛虫(Giardia)和牛轮状病毒(Bovine Rotavirus,BRV),测序结果显示样品中鉴定的为相同的病原。结果表明,刚察县吉尔孟乡牧场犊牛的腹泻是由隐孢子虫,贾第鞭毛虫和牛轮状病毒的混合感染造成的,该研究为犊牛腹泻的病原学研究和流行病学调查提供了参考数据,为针对性地对病原性致病因素的对症对因治疗和辅助支持治疗提供了参考。There was an yak diarrhea disease occurring in a pasture in Giermeng Town of Gangcha County of Qinghai Province.For the rapid diagnosis of the cause of yak diarrhea,and timely prevent and treat the diarrhea,eight yak diarrhea samples were collected,the microscopic examination method and PCR molecular method were used for identification and analysis.The results showed that the pathogens causing diarrhea in this pasture were Cryptosporidium,Giardia and Bovine Rotavirus.The results showed that:the yak diarrhea in Gangcha Giermeng Town pasture was caused by a mixed infection of Cryptosporidium,Giardia and Bovine Rotavirus.This study provided reference data for the pathogenic research and epidemiological investigation of yak diarrhea,and also provided reference for symptomatic treatment and auxiliary supportive treatment of pathogens.

关 键 词：犊牛 腹泻 隐孢子虫 贾第虫 轮状病毒 分子鉴定 

分 类 号：S823.85[农业科学—畜牧学]