四川德阳地区血小板捐献者HPA-1~6w、HPA-15及HPA-32bw~35bw多态性研究  

The polymorphism of HPA-1-6w, HPA-15 and HPA-32bw-35bw in platelet donors from Deyang, Sichuan

作　　者：吕启露 廖秀云[3] 纪欣[1,2] 袁丽 钱立琼[3] 舒世春 陈强 王珏[1,2] LV Qi-lu;LIAO Xiuyun;JI Xin;YUAN Li;QIAN LIqiong;SHU Shichun;CHEN Qiang;WANG Jue(Institute of Blood,Transfusion,Chinese Academy of Medical Sciences and Peking Union Medical College,Chengdu 610025,China;Key Laboratory of Adverse Reactions of Blood Transfusion y Chinese Academy of Medical Sciences;Deyang Central Blood Station)

机构地区：[1]中国医学科学院北京协和医学院输血研究所 [2]中国医学科学院输血不良反应重点实验室 [3]德阳市中心血站

出　　处：《中国输血杂志》2022年第2期149-153,共5页Chinese Journal of Blood Transfusion

基　　金：中国医学科学院健康与科技创新工程(2016-I2M-3-024);中央公益科研院所基本科研业务费(2018PT32016);德阳市科技支撑计划(2017SZ028)。

摘　　要：目的了解四川德阳地区血小板捐献者HPA-1~6w、15及32bw~35bw的多态性,并评估32bw~35bw位点是否纳入血小板库常规检测。方法采用PCR-测序分型(SBT)方法,对205名德阳地区血小板捐献者的HPA-1~6w、15及32~35bw位点测序后,采用直接计数法计算等位基因频率,与中国南北方人群及澳大利亚、日本人群HPA-1~6和15等位基因频率比较,并在百万中国人基因数据库(CMDB)和基因组聚合数据库(genomAD)中查询以获得相应的HPA位点多态性信息,运用GraphPad Prism 9软件与本研究数据做卡方检验。结果本组血小板捐献者的HPA-1b、2b、3b、5b、6bw和15b等位基因频率分别为0.005(2/410)、0.037(15/410)、0.471(193/410)、0.020(8/410)、0.010(4/410)和0.461(189/410), 32bw~35bw和HPA-4中b等位基因未检出。德阳血小板捐献者HPA-1b等位基因频率与中国北方、澳大利亚人群和genomAD全球人群标本中有差异(0.005 vs 0.014 vs 0.145 vs 0.122,P<0.05);HPA-2b等位基因频率与日本人群和genomAD中全球人群标本有差异(0.037 vs 0.120 vs 0.100,P<0.05);HPA-5b和HPA-6bw等位基因频率与genomAD全球人群标本有差异(分别为0.020 vs 0.089,0.010 vs 0.000 008,P<0.05)。结论德阳地区血小板捐献者HPA-1~6w和HPA-15多态性具有中国南方人群的特征,HPA-32bw~35bw这几个位点的频率极低,可以排除纳入血小板捐献者的常规检测。Objective To explore the polymorphism of HPA-1-6w, HPA-15 and 32bw-35bw in platelet donors in Deyang, Sichuan, and estimate whether to include the detection of 32bw-35bw in the platelet bank. Methods Polymerase chain reaction with sequenced based typing(PCR-SBT) was used to sequence the HPA-1-6w, HPA-15 and 32bw-35bw loci of 205 platelet donors in Deyang. Allele frequencies were calculated by the direct counting method. The frequencies of HPA-1-6 and 15 alleles in northern and southern Chinese, Japanese and Australian population were compared, and those HPA loci and HPA-32bw-35bw were searched in the Chinese Millionome Database(CMDB) and genomAD to obtain the polymorphism data. Then the Chi-square test was performed with the data of this study through GraphPad Prism 9 software. Results The allele frequencies of HPA-1b, 2b, 3b, 5b, 6bw and HPA-15b were 0.005(2/410), 0.037(15/410), 0.471(193/410), 0.020(8/410), 0.010(4/410) and 0.461(189/410), respectively, b allele of HPA-32bw-35bw and HPA-4 was not detected. Statistical significance was observed between the HPA-1b allele frequency of this study and northern Chinese, Australian population and genomAD global population sample(P< 0.05, 0.005 vs 0.014 vs 0.145 vs 0.122). The frequency of HPA-2b alleles in this study, Japanese population and genomAD global population samples was 0.037 vs 0.120 vs 0.100, with statistical difference(P<0.05). Comparison of HPA-5b and HPA-6bw allele frequencies with those of genomAD global population showed a statistical difference(P<0.05, 0.020 vs 0.089 and 0.010 vs 0.000 008, respectively). Conclusion The polymorphisms of HPA-1-6w and HPA-15 of donors in Deyang has characteristics of the southern Chinese. The frequencies of HPA-32bw-35bw were extremely low, which could be excluded from the platelet bank in Deyang.

关 键 词：人类血小板抗原 多态性 血小板捐献者 德阳 PCR-基因测序 

分 类 号：R457.11[医药卫生—治疗学] R392.2[医药卫生—临床医学]