中山地区RhD阴性血型标本D^(el)表型基因分型研究  

Molecular basis of D^(el) phenotype in RhD negative population of Zhongshan

作　　者：陈勇高[1] 吴少梅[1] 易冰[2] 赖世晓 CHEN Yonggao;WU Shaomei;YI Bing;LAI Shixiao(Department of Blood Transfusion,Zhongshan People's Hospital,Zhongshan 528400,China;Center for Molecular Diagnosis,Zhongshan People's Hospital,Zhongshan 528400,China.)

机构地区：[1]中山市人民医院输血科,广东中山528400 [2]中山市人民医院分子诊断中心,广东中山528400

出　　处：《中国输血杂志》2022年第2期157-161,共5页Chinese Journal of Blood Transfusion

基　　金：中山市科技计划项目(2017B1057)。

摘　　要：目的探索中山地区RhD阴性血型标本D^(el)型表型及基因分型特征,提高D^(el)型诊断水平。方法 2017年12月~2019年2月在102例初筛为RhD阴性标本中进行RHCE分型与PCR-SSP基因分型,以抗球卡法(IAT)确认RhD阴性95例,吸收放散试验鉴定D^(el)型28例,最后结合国内数据分析D^(el)型表型与基因分型特征,总结中山地区D^(el)型特征和地区特异性。结果血清学试验初筛RhD阴性的102例血型标本中,RhD阴性95例;确定D^(el)RHD 1227A型28例,未见其它D^(el)型等位基因;其中20例RHCE抗原分型为Ccee (71.4%),8例为CCee (28.6%),与国内其它地区无差异性。中山地区RhD阴性D^(el)分布频率29.5%(28/95),并存在地区差异性:与南昌,浙江,武汉无差异;而与上海,台湾,福州有差异。结论本次调查中,D^(el)表型与Ce单体型密切相关,与国内其它地区无差异性;D^(el)型在RhD阴性分布频率29.5%,存在地区差异性;D^(el)型以RHD1227A为主要等位基因。Objective To conduct serological and molecular study of D^(el) type in RhD-negative donor population in Zhongshan area, so as to improve the diagnosis of D^(el) type. Methods A total of 102 initially RhD-negative samples, collected from December 2017 to February 2019, were classified by RHCE and PCR-SSP genotyping. And 95 cases of truly negative RhD were confirmed by IAT, 28 cases of D^(el) type were identified by absorption and elution test. The phenotype and genotyping characteristics of D^(el) type in Zhongshan area were summarized based on domestic data of relative literature.Results Among 102 initially RhD-negative samples by serological test, 95 were truely RhD-negative, 28 were D^(el) RHD 1227 A without any other D^(el) allele. Among them, RHCE antigen type were Ccee in 20(71.4%) cases, CCee in 8(28.6%), with no difference in comparison with other regions in China. The frequency of D^(el) in RhD-negative blood donors was 29.5%(28/95), with difference between Shanghai, Taiwan, and Fuzhou, but no difference between Nanchang, Zhejiang, and Wuhan.Conclusion The study showed that the D^(el) phenotype was closely related to Ce haplotype, and has no difference with other regions in China. The frequency of D^(el) type in RhD negative donors was 29.5%, with regional differences. RHD1227 A was the main allele of D^(el).

关 键 词：RHD阴性 D^(el)表型 RHCE分型 PCR-SSP技术 

分 类 号：R457.11[医药卫生—治疗学]