街头采血纸板法ABO血型检测条件优化探讨  

Optimization of detection conditions of pre-donation ABO blood type screening by sliding methods in fixed blood donation sites

作　　者：李丹[1] 吕永磊[1] 冯利利 王顼 张晓天 刘亚娟 许盼盼[1] 赵磊[1] LI Dan;LYU Yonglei;FENG Lili;WANG Xu;ZHANG Xiaotian;LIU Yajuan;XU Panpan;ZHAO Lei(Henan Red Cross Blood Center,Zhengzhou 4500521 China)

机构地区：[1]河南省红十字血液中心,河南郑州450052

出　　处：《中国输血杂志》2022年第2期161-163,共3页Chinese Journal of Blood Transfusion

摘　　要：目的探讨纸板法ABO血型检测关键点量化值,为街头采血纸板法ABO血型正定型检测操作方法标准化提供借鉴。方法对纸板法ABO血型检测过程中试剂血清用量、全血红细胞量以及反应时间等条件的改变对检测凝集程度和结果造成的影响进行比较,初步确定街头采血纸板法ABO血型检测关键点量化值。按照有关量值采用纸板法检测献血者ABO正定型,其中包括正常A、B、O、AB型,以及A亚型、AB亚型共310份。将检测结果与血型仪微板法正定型结果进行比对,结果不一致的通过试管法复核,对疑似ABO亚型结果采用血清学和分子生物学方法确认。结果纸板法ABO血型检测关键点量化值是:2滴试剂血清、5~10μL全血和3分钟反应时间。310份献血者标本ABO正定型比对实验符合率为100%。结论按照纸板法ABO血型检测量化值操作,可以有效规范街头采血ABO血型检测关键点,进一步满足血站初筛ABO血型检测安全需求。Objective To explore the quantitative value of key points for ABO blood group initial screening in fixed blood donation sites, so as to provide reference for standardized testing process of sliding method. Methods Several groups of experiments were carried out to illustrate the optimal conditions, including the serum dosage of monoclonal reagent, red blood cell dosage of blood sample, and reaction time in ABO blood group initial screening, by sliding method, and the quantitative value of key points of sliding method was preliminarily determined. Blood typing tests of 310 blood donor samples including type A, B, O, AB, subtype A and subtype AB were conducted to evaluate the effects of quantitative value of key points in the initial screening procedure. The test tube method would be conducted if the results are inconsistent with the fully automated blood grouping analyzer. The ABO subtypes suspected are identified by serological and molecular biological methods. Results The quantitative value of key points in initial screening procedure of sliding methods was as follows: 2 drops of reagent serum, 5-10 μL of whole blood and 3 minutes of reaction time. The concordance rate of ABO blood type screening comparison experiment in 310 blood donors was 100%. Conclusion ABO blood group initial screening by sliding method with quantitative value can effectively standardize the pre-donation blood type screening in fixed blood donation sites, and can meet the requirements of ABO blood group initial screening.

关 键 词：街头采血 ABO血型初筛 纸板法 血液安全 

分 类 号：R457.11[医药卫生—治疗学]