Functional characterization of a novel, highly expressed ion-driven sugar antiporter in the thoracic muscles of Helicoverpa armigera  

作　　者：Yi-Yang Yuan Yu-Cui Xin Jian-Li Han Yu-Han Zhao Shi-Ming Han Zi-Yan Nangong Xin Chen Zhu-Cheng Wang Mei Li Xing-Hui Qiu 

机构地区：[1]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,China [2]College of Life Sciences,University of Chinese Academy of Sciences,Beijing,China [3]College of Life Sciences,Cangzhou Normal University,Cangzhou,Jiangsu Province,China [4]College of Plant Protection,Hebei Agricultural University,Baoding,Hebei Province,China

出　　处：《Insect Science》2022年第1期78-90,共13页昆虫科学（英文版）

基　　金：This work is supported by grants from National Natural Science Foundation of China(grant numbers 31601644 and 31970453);the State Key Laboratory of Integrated Management of Pest Insects and Rodents(grant numbers ChineselPM 1607 and ChineselPMl717).

摘　　要：Sugar transporters(STs),which mainly mediate cellular sugar exchanges,play critical physiological roles in living organisms,and they may be responsible for sugar exchanges among various insect tissues.However,the molecular and physiological functions of insect STs are largely unknown.Here,16 STs of Helicoverpa armigera were identified.A phylogenetic analysis classified the putative HaSTs into 12 sub-families,and those identified in this study were distributed into 6 sub-families.Real-time polymerase chain reaction indicated that the 16 HaSTs had diverse tissue-specific expression levels.One transporter,HaST10,was highly expressed in thoracic muscles.A functional study using a Xenopus oocyte expression system revealed that HaST10 mediated both H+-driven trehalose and Na+-driven glucose antiport activities with high transport efficiency and low affinity levels.A HaST10 knockout clearly impaired the performance of H.armigera.Thus,HaST10 may participate in sugar-supply regulation and have essential physiological roles in H.armigera.

关 键 词：CRISPR/Cas9 GLUCOSE Helicoverpa armigera sugar transporter TREHALOSE thoracic muscles 

分 类 号：Q96[生物学—昆虫学]