Juvenile hormone regulates photoperiod-mediated male reproductive diapause via the methoprene-tolerant gene in the ladybeetle Harmonia axyridis  

作　　者：Qiao Gao Bei Li Bing-Xin Wei Wen Liu Pan Wang Jia-Lu Wang Xing-Miao Zhou Xiao-Ping Wang 

机构地区：[1]Hubei Key Laboratory of Insect Resources Utilization and Sustainable Pest Management,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,430070,China [2]Department of Plant Protection,Wuhan Vegetable Research Institute,Wuhan,430065,China

出　　处：《Insect Science》2022年第1期139-150,共12页昆虫科学（英文版）

基　　金：This study was funded by the National Natural Science Foundation of China(grant no.31701842);Hubei Provincial Natural Science Foundation of China(grant no.2019CFB475).

摘　　要：Juvenile hormone (JH) absence induces photoperiod-mediated reproductive diapause, which is characterized by reproductive cessation. Although the role of methoprene-tolerant (Met)-mediated JH signaling in photoperiod-mediated female reproduction has been well documented, its role in male reproduction remains unclear. In this study, we investigated the role of JH in regulating photoperiod-mediated development of the male internal reproductive system (IRS) in the predatory ladybeetle Harmonia axyridis (Pallas). In a previous study, we found that adult male H. axyridis reared under either a short-day (SD) or long-day (LD) photoperiod had obvious differences in IRS development, but we were unable to identify the regulators of male reproductive diapause. In this study, we found that beetles reared under an SD photoperiod had significantly lower JH titer and a relatively undeveloped male IRS compared with those reared under an LD photoperiod. Additionally, application of the JH analog (JHA) methoprene promoted IRS development. Furthermore, Met knockdown strongly blocked JH signaling in males reared under the LD photoperiod, thereby slowing IRS development. Moreover, exogenous JHA did not reverse the suppressed development of the male IRS caused by Met knockdown. These results indicate that photoperiod regulates male IRS development in H. axyridis through a conserved Met-dependent JH signaling pathway.

关 键 词：Harmonia axyridis juvenile hormone male reproductive diapause methoprene-tolerant PHOTOPERIOD RNAI 

分 类 号：Q96[生物学—昆虫学]