Appraisal of MsepCSP14 for chemosensory functions in Mythimna separata  

作　　者：Aneela Younas Muhammad Irfan Waris Muhammad Shaaban Muhammad Tahir ul Qamar Man-Qun Wang 

机构地区：[1]College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China [2]Department of Soil Science,Faculty of Agricultural Sciences and Technology,Bahauddin Zakariya University,Multan,Pakistan [3]State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning,530005,China

出　　处：《Insect Science》2022年第1期162-176,共15页昆虫科学（英文版）

基　　金：This study was funded by the National Key Research and Development Program of China(2017YFE0113900);Special Technical Innovation of Hubei Province(2017ABA146).

摘　　要：Chemosensory proteins (CSPs) have great contributions in performing diverse functions in insects. However, physiological appraisal of chemosensory protein genes still remains elusive in insects. We studied expression patterns and binding affinities of MsepCSP14, a chemosensory protein, in Mythimna separata. The distinct functions of MsepCSP14 were validated by employing different molecular techniques. The MsepCSP14 had high resemblance of sequence with chemosensory proteins of other insect family members. The MsepCSP14 expression was higher in antennal tissues of females than other tissues. Fluorescence binding assay validated that binding of nine out of 21 ligands to MsepCSP14 was higher at pH 7.4 than at pH 5.0. Three dimensional modeling (3D) and docking analysis predicted that amino acid residues of MsepCSP14 were involved in binding of compounds, and behavior assay displayed that adults of M. separata considerably responded to four volatiles from compounds demonstrating strong binding ability to MsepCSP14. Results of the present study suggest that MsepCSP14 is likely to mediate chemosensory functions in M. separata.

关 键 词：CHEMORECEPTION expression pattern fluorescent binding OLFACTION oriental army worm Y-tube 

分 类 号：Q96[生物学—昆虫学]