Resistance and fitness costs in diamondback moths after selection using broflanilide, a novel meta-diamide insecticide  

作　　者：Xi Sun Rui Wei Linhong Li Bin Zhu Pei Liang Xiwu Gao 

机构地区：[1]Department of Entomology,China Agricultural University,Beijing,100193,China

出　　处：《Insect Science》2022年第1期188-198,共11页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31371956,31572023 and 31772186).

摘　　要：The diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera: Plutellidae) is an insect pest found around the world that feeds on cruciferous crops. The DBM has become resistant to most insecticides in current use in the field. Broflanilide is a novel meta-diamide insecticide that binds to a new site on the γ-aminobutyric acid receptor and very efficiently protects against most pests in the order Lepidoptera, including DBM. In this study, the resistance of a laboratory-bred susceptible strain of DBM to broflanilide and the fitness costs posed by broflanilide to the DBM were evaluated. The DBM had no obvious resistance to broflanilide after 10 generations of selection. The realized heritability h2 was 0.033, suggesting a low risk of resistance developing in this strain. The F10 generation had no cross-resistance to the insecticides abamectin and endosulfan (which target the γ-aminobutyric acid receptor) and chlorantraniliprole (which targets a non-γ-aminobutyric acid receptor). The specific activities of important detoxification enzymes (cytochrome P450 monooxygenase, esterase, and glutathione S-transferase) were not obviously altered. However, the larval stage was prolonged and the adult stage was shortened significantly in F11 generation than the F0 generation. The total preoviposition period TPOP significantly prolonged 1.90 d in F11 generation. The fitness value Rf (0.93) was lower for the F11 generation than the F0 generation. The results indicated that long-term exposure to broflanilide exerts clear fitness costs in the DBM. This information will be useful in identifying reasonable broflanilide application guidelines for managing broflanilide resistance in the DBM.

关 键 词：broflanilide CROSS-RESISTANCE detoxifying enzymes fitness cost Plutella xylostella 

分 类 号：S435[农业科学—农业昆虫与害虫防治]