多重PCR技术可鉴定杂交水稻种子纯度  

The Seed Purity of Hybrid Rice Could be Identified by Multiple PCR Technology

作　　者：马增凤[1] 韦敏益 黄大辉[1] 张月雄[1] 刘驰[1] 罗同平[1] 李振经[1] 秦钢[1] Ma Zengfeng;Wei Minyi;Huang Dahui;Zhang Yuexiong;Liu Chi;Luo Tongping;Li Zhenjing;Qin Gang(Guangxi Key Laboratory of Rice Genetics and Breeding,Rice Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,530007)

机构地区：[1]广西农业科学院水稻研究所广西水稻遗传育种重点实验室,南宁530007

出　　处：《分子植物育种》2022年第2期442-447,共6页Molecular Plant Breeding

基　　金：广西科技重大专项(桂科AA17204050-2)、广西科技重大专项(桂科AA17202044;桂科AA17204049);广西农业科学院水稻研究所自选项目(桂农科水稻ZX-05);广西科技重点研发项目(桂科AB16380079;桂科AB18221043)共同资助。

摘　　要：为建立一种利用多重PCR技术对水稻多个基因位点进行同步检测的方法,本研究以‘丰田优553’、‘吉丰优3550’及其杂交种子为试验材料,根据不同SSR分子标记的扩增片段差异,筛出亲本间差异明显的SSR分子标记,然后设计多重PCR鉴定杂交水稻种子的纯度,并与普通PCR的鉴定结果进行对比分析,验证多重PCR鉴定杂交水稻种子纯度实用性。结果显示,共筛选到48对SSR分子标记,其中‘丰田1A’与‘桂恢553’之间的差异性引物有23对;‘吉丰A’与‘广恢3550’之间的差异性引物有24对。根据PCR扩增片段大小的差异,对两个杂交组合设计的两组双重PCR的纯度鉴定发现,普通PCR的鉴定结果与多重PCR的结果一致。说明多重PCR能够对多个水稻基因位点进行同步检测,为杂交水稻种子纯度的鉴定提供技术参考。In order to establish a method for simultaneous detection of multiple gene loci in rice by using multiple PCR technology, hybrid seeds of ’Fengtian You 553’ and ’Jifeng You 3550’ and their parents were used as experimental materials in this study. Based on the differences in the amplified fragments of different SSR molecular markers, SSR molecular markers with significant differences between parents were screened out. Then, multiple PCR was designed to identify the purity of hybrid rice seeds, and the results were compared with those obtained by ordinary PCR, so as to verify the practicability of multiple PCR in identifying hybrid rice seed purity. The results showed that a total of 48 pairs of SSR molecular markers were screened, among which 23 pairs of primers were different between ’Fengtian 1 A’ and ’Guihui 553’. There were 24 pairs of different primers between ’Jifeng A’ and’Guanghui 3550’. According to the difference of PCR amplification fragment size, the purity identification of two groups of double PCR designed by two hybrid combinations showed that the identification results of common PCR were consistent with the results of multiple PCR. The results showed that multiple PCR could detect multiple rice gene loci synchronously and provide technical reference for the identification of hybrid rice seed purity.

关 键 词：多重PCR 纯度鉴定 杂交水稻 

分 类 号：S511[农业科学—作物学]