基于正交设计的露薇花组培苗增殖及瓶内开花  

Proliferation and Flowering in Vitro of Tissue Culture Seedlings of Lewisia cotyledon Based on Orthogonal Design

作　　者：马村艺 张黎[1] Ma Cunyi;Zhang Li(College of Agriculture,Ningxia University,Yinchuan,750021)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《分子植物育种》2022年第2期494-498,共5页Molecular Plant Breeding

基　　金：宁夏回族自治区重点研发计划项目(2019BBF02011)资助。

摘　　要：以露薇花组培苗为试验材料,采用L_(9)(3^(3))正交试验方法,在MS基本培养基中添加不同浓度的蔗糖、6-BA和NAA,以期筛选露薇花增殖的最适条件;添加不同浓度的6-BA,NAA和GA_(3),筛选最适露薇花瓶内开花的培养条件。结果表明,露薇花无菌苗最佳增殖条件为MS+0.5 mg/L NAA+1.0 mg/L 6-BA (蔗糖浓度30 g/L),最高增殖倍数为3.17倍,最适开花条件为MS+1.0 mg/L 6-BA+3.0 mg/L GA_(3)+0.5 mg/L NAA,平均开花率可达70%。本研究为露薇花的高效繁殖和试管花卉的开发提供了理论依据和技术支持。Using the orthogonal design of three factors and three levels L_(9)(3^(3)) method, and different concentrations of sucrose, 6-BA and NAA were added to MS basic medium in order to screen the optimal proliferation conditions for the aseptic seedling of Lewisia cotyledon. Different concentrations of 6-BA, NAA and GA_(3) were added to MS basic medium to select the optimal conditions for flowering in vitro. The results showed that MS+0.5 mg/L NAA+1.0 mg/L 6-BA(sucrose concentration 30 g/L) was the best proliferation condition, the highest proliferation multiple was 3.17 times, the most suitable flowering in vitro condition was MS+1.0 mg/L 6-BA+3.0 mg/L GA_(3)+0.5 mg/L NAA, and the average flowering rate could reach 70%. This research provides theoretical basis and technical guidance for the efficient propagation and the development of test tube flower in Lewisia cotyledon.

关 键 词：露薇花(Lewisia cotyledon) 组织培养 正交试验设计 瓶内开花 

分 类 号：S682.33[农业科学—观赏园艺]