基于HPLC多指标成分测定及指纹图谱多模式识别方法的北细辛质量分析  

Quality assessment of Asarum heterotropoides var. mandshuricum based on HPLC multi-component determination and multiple pattern recognition method of fingerprint

作　　者：李妍 何文媛 王康宇 白羽辛 高文义 贡济宇[1] LI Yan;HE Wen-yuan;WANG Kang-yu;BAI Yu-xin;GAO Wen-yi;GONG Ji-yu(College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117

出　　处：《中草药》2022年第1期238-243,共6页Chinese Traditional and Herbal Drugs

基　　金：国家中药标准化项目(ZYBZH-Y-JL-25);古代经典名方颗粒剂的开发研究(SW-KF105);吉林省科技发展计划项目(20170204028YY)。

摘　　要：目的通过HPLC多成分含量测定与指纹图谱的多模式化学识别方法对北细辛Asarumheterotropoidesvar.mandshuricum进行不同产地质量分析研究,为北细辛药材质量评价提供参考。方法收集了4个产区的18批细辛样品,采集HPLC指纹图谱并进行3种有效成分的含量测定,通过聚类分析(cluster analysis,CA)、主成分分析(principal component analysis,PCA)、正交偏最小二乘法判别分析(orthogonal partial least squares discriminant analysis,OPLS-DA)分别探讨不同产区北细辛的质量。结果建立了北细辛药材的指纹图谱,确定共有峰17个,指认了3个共有峰。甲基丁香酚、芝麻脂素、细辛脂素的质量分数分别为1.76~32.98、1.03~6.05、1.59~6.91mg/g。CA可将4个产地北细辛药材聚为2类,PCA结果提取了2个主成分,OPLS-DA能有效地将2大产区药材明显地区分开。结论通过北细辛HPLC含量测定与指纹图谱建立的多模式识别法全面评价了北细辛的质量,为北细辛药材质量评价提供依据,可作为北细辛质量控制的有效方法。Objective To analyze the quality of Asarum heterotropoides var. mandshuricum from different areas by multiple chemical pattern recognition based on HPLC fingerprint and multi-component content, so as to provide a reference for the quality evaluation of A. heterotropoides var. mandshuricum. Methods A total of 18 batches of A. heterotropoides var. mandshuricum samples from four producing areas were collected, HPLC fingerprints were collected and the content of three active ingredients was determined. The quality of A. heterotropoides var. mandshuricum in different areas was explored through CA, PCA and OPLS-DA methods. Results The fingerprint of A. heterotropoides var. mandshuricum was established, and three common peaks were identified. The mass fractions of methyl eugenol, sesamin and asarinin were 1.76—32.98, 1.03—6.05 and 1.59—6.91 mg/g,respectively. CA method could group the four origins of A. heterotropoides var. mandshuricum medicinal materials into two categories. PCA results extracted two principal components. The medicinal materials of the two major producing areas were clearly distinguished by OPLS-DA. Conclusion The multi-pattern recognition method based on the establishment of the HPLC fingerprint and the determination of the content comprehensively evaluates the quality of A. heterotropoides var. mandshuricum. It provides a basis for the quality evaluation of medicinal materials and can be used as an effective method for quality control of A.heterotropoides var. mandshuricum.

关 键 词：细辛 HPLC 指纹图谱 化学模式识别 质量评价 甲基丁香酚 芝麻脂素 细辛脂素 

分 类 号：R286.2[医药卫生—中药学]