藏药亚吉玛(长梗金腰)醋酸乙酯部位的化学成分研究  

Isolation and identification of chemical constituents from Tibetan medicine “Ya-Ji-Ma”(Chrysosplenium axillare)

作　　者：俞志凌 张驰 甘慧琴 邓文赞 曹岚 钟国跃 黄四碧[2] 任刚 YU Zhi-ling;ZHANG Chi;GAN Hui-qin;DENG Wen-zan;CAO Lan;ZHONG Guo-yue;HUANG Si-bi;REN Gang(Research Center of Natural Resources of Chinese Medicinal Materials and Ethnic Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学中药资源与民族药研究中心,江西南昌330004 [2]江西中医药大学附属医院,江西南昌330004

出　　处：《中草药》2022年第2期354-361,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81873086);江西省自然科学基金面上项目(20181BAB205076);国家重点研发计划资助(2019YFC1712300);江西中医药大学校级研究生创新专项资金资助项目(JZYC20S40)。

摘　　要：目的研究藏药亚吉玛(长梗金腰Chrysosplenium axillare)醋酸乙酯部位的化学成分。方法采用HP-20大孔吸附树脂、Sephadex LH-20凝胶等柱色谱以及制备高效液相技术进行化学成分分离,采用NMR和HR-ESI-MS等光谱方法进行化合物结构鉴定。结果分离得到17个高度氧化的黄酮醇衍生物及4个三萜类化合物,其结构分别鉴定为5,6,4’-三羟基-3,7,3’-三甲氧基黄酮-6-O-β-D-葡萄糖苷(1)、5,6,3’-三羟基-3,7,4’-三甲氧基黄酮-6-O-β-D-葡萄糖苷(2)、chrysosplenoside H(3)、5-O-demethylapulein (4)、5,3’-二羟基-3,6,7,4’-四甲氧基黄酮(5)、5,2’-二羟基-3,7,4’,5’-四甲氧基黄酮(6)、chrysosplenol C (7)、5,2’,5’-三羟基-3,7,4’-三甲氧基黄酮(8)、axillarin (9)、brickellin (10)、槲皮素(11)、芦丁(12)、isorientin (13)、chrysosplenosideE (14)、槲皮素3-O-β-D-葡萄糖苷(15)、5,6,3’,4’-四羟基-3,7-二甲氧基黄酮-6-O-β-D-葡萄糖苷(16)、tomentin(17)、β-peltobo1toykinolic acid(18)、3α,16α-二羟基-4,4,9,14-四甲基-(9β,10α)-19-去甲孕酮-5-烯-11,20-二酮(19)、3α,16α,20,25-四羟基-(10α)-葫芦素-5-烯-11,22-二酮(20)、6α,23α-环氧-3β,20β-二羟基-10αH,23βH-葫芦素-5,24-二烯-11-酮(21)。结论化合物2、4~6、8、9、19~21为首次从金腰属植物中分离得到,其余化合物均为首次从长梗金腰中分离得到。Objective To isolate and identify the chemical constituents from the ethyl acetate fraction of the Tibetan medicine Chrysosplenium axillare. Methods HP-20 macroporous adsorption resin, Sephadex LH-20 gel column chromatography and preparative high performance liquid chromatography were used to separate the chemical constituents. The structures of all isolates were elucidated by spectroscopic methods, including NMR and HR-ESI-MS. Results Seventeen highly oxidized flavonol derivatives and four triterpenoids were isolated and their structures were identified as 5,6,4′-trihydroxy-3,7,3′-trimethoxyflavone-6-O-β-D-glucoside(1), 5,6,3′-trihydroxy-3,7,4′-trimethoxyflavone-6-O-β-D-glucoside(2), chrysospenoside H(3),5-O-demethyllapulein(4), 5,3′dihydroxy-3,6,7,4′-tetramethoxyflavone(5), 5,2′-dihydroxy-3,7,4′,5′-tetramethoxyflavone(6),chrysosplenol C(7), 5,2′,5′-trihydroxy-3,7,4′-trimethoxyflavone(8), axillarin(9), brickellin(10), quercetin(11), rutin(12), isorientin(13), chrysosplenoside E(14), quercetin-3-O-β-D-glucoside(15), 5,6,3′,4′-tetrahydroxy-3,7-dimethoxyflavone-6-O-β-D-glucoside(16), tomentin(17), β-peltoboykinolic acid(18), 3α,16α-dihydroxy-4,4,9,14-tetramethyl-(9β,10α)-19-norpregn-5-ene-11,20-dione(19), 3α,16α,20,25-tetrahydroxy-(10α)-cucurbit-5-ene-11,22-dione(20), 16α,23α-epoxy-3β,20β-dihydroxy-10αH,23βH-cucurbit-5,24-dien-11-one(21). Conclusion It is the first report for the compounds 2, 4—6, 8, 9, 19—21 identified in the genus Chrysosplenium. The other compounds were isolated from C. axillare for the first time.

关 键 词：长梗金腰 高度氧化黄酮醇 齐墩果烷型三萜 葫芦烷型三萜 降三萜 

分 类 号：R284.1[医药卫生—中药学]