人参-川芎有效成分通过PPARγ/PGC-1α/UCP1通路调控白色脂肪棕色化的机制研究  

Mechanism research of effective ingredients of Ginseng Radix et Rhizoma and Chuanxiong Rhizoma on regulating white adipose browning by PPARγ/PGC-1α/UCP1 pathway

作　　者：乔羽 张京春[2] 刘玥[2] 马林沁 毛婷 孙欣丽 QIAO Yu;ZHANG Jing-chun;LIU Yue;MA Lin-qin;MAO Ting;SUN Xin-li(Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]北京中医药大学东方医院,北京100078 [2]中国中医科学院西苑医院,北京100091

出　　处：《中草药》2022年第2期478-483,共6页Chinese Traditional and Herbal Drugs

基　　金：中央高校基本科研业务费专项资金资助(2020-JYB-XJSJJ-052);国家自然科学基金资助项目(81573817)。

摘　　要：目的研究人参、川芎有效成分对慢性间歇性低氧干预肥胖胰岛素抵抗诱导动脉粥样硬化过程中白色脂肪棕色化的调节作用机制。方法使用ApoE;小鼠建立动物模型,给予振源胶囊(人参总皂苷)和磷酸川芎嗪片(川芎嗪)干预8周,记录小鼠体质量并监测血糖;使用比色法检测小鼠血清中总胆固醇(totalcholesterol,TC)、三酰甘油(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白(low density lipoprotein choleste rol,LDLC)水平;取小鼠腹腔脏器及腹主动脉周围的脂肪组织,采用Western blotting法检测脂肪组织中过氧化物酶体增殖物激活受体γ(pero xisome proliferator-activated receptorγ,PPARγ)、过氧化物酶体增生物激活受体γ辅激活子-1α(peroxisome proliferatoractivated receptorγco-activator-1α,PGC-1α)、解偶联蛋白1 (uncoupling protein 1,UCP1)蛋白表达。结果与空白对照组和常氧对照组相比,模型组小鼠体质量、血糖、LDL-C水平明显升高(P<0.05、0.01);与模型组相比,人参-川芎有效成分高剂量组血糖、TG、LDL-C水平显著降低(P<0.05、0.01),脂肪组织中PPARγ、PGC-1α以及UCP1蛋白表达水平明显升高(P<0.05、0.01)。结论人参、川芎有效成分能够通过PPARγ/PGC-1α/UCP1信号通路促进白色脂肪棕色化,进而降低慢性间歇性低氧复合肥胖胰岛素抵抗诱导的动脉粥样硬化小鼠体质量、血糖、LDL-C、TG水平发挥抗动脉粥样硬化的作用。Objective To investigate the mechanism of effective ingredients of Renshen(Ginseng Radix et Rhizoma)and Chuanxiong(Chuanxiong Rhizoma)on regulating white adipose browning in the process of chronic intermittent hypoxia intervention in obesity and insulin resistance-induced atherosclerosis.Methods Apo E-/-mice were used to establish an animal model,and Zhenyuan Capsules(振源胶囊,total ginsenosides)and Ligustrazine Phosphate Tablets(磷酸川芎嗪片,tetramethylpyrazine)were administered for eight weeks.Body weight of mice was recorded and blood glucose was monitored.Colorimetric methods were used to detect total cholesterol(TC),triglyceride(TG),high density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)in serum.The adipose tissue surrounding abdominal organs and aorta was harvested,Western blotting was used to detect the protein expressions of peroxisome proliferator-activated receptorγ(PPARγ),peroxisome proliferator-activated receptorγco-activator-1α(PGC-1α)and uncoupling protein 1(UCP1).Results Compared with blank and normoxic control groups,body weight,blood glucose and LDL-C levels in model group were significantly increased(P<0.05,0.01).Compared with model group,blood glucose,LDL-C and TG levels were significantly decreased in effective ingredients of Ginseng Radix et Rhizoma and Chuanxiong Rhizoma high-dose group(P<0.05,0.01),PPARγ,PGC-1αand UCP1 protein expressions in adipose tissue were significantly increased(P<0.05,0.01).Conclusion Effective ingredients of Ginseng Radix et Rhizoma and Chuanxiong Rhizoma can promote the adipose tissue browning via PPARγ/PGC-1α/UCP1 signaling pathway and reduce body weight,blood glucose,LDL-C and TG levels,and thus exerting antiatherosclerotic effects.

关 键 词：人参-川芎有效成分 人参总皂苷 川芎嗪 肥胖 白色脂肪棕色化 胰岛素抵抗 动脉粥样硬化 慢性间歇性低氧 过氧化物酶体增殖物激活受体γ 过氧化物酶体增生物激活受体γ辅激活子-1α 解偶联蛋白1 

分 类 号：R285.5[医药卫生—中药学]