尿激酶对深静脉血栓大鼠体内炎症物质和血栓调节蛋白的影响  

Effect of urokinase on inflammatory substances and thrombomodulin in rats with deep vein thrombosis

作　　者：叶玲 吕永烨 刘蓉 Ye Ling;Lyu Yongye;Liu Rong(Department of Vascular Surgery,Qingdao Hospital of Traditional Chinese Medicine(Qingdao Haici Hospital),Qingdao 266000,China;Department of Cardiac Surgery,Qingdao Municipal Hospital,Qingdao 266011,China)

机构地区：[1]青岛市中医医院(青岛市海慈医院)血管外科,青岛266000 [2]青岛市市立医院心外科,青岛266011

出　　处：《中国医师杂志》2022年第2期270-274,共5页Journal of Chinese Physician

摘　　要：目的探讨尿激酶对深静脉血栓(DVT)大鼠体内炎症物质和血栓调节蛋白(TM)的影响机制。方法建立深静脉血栓大鼠模型,把30只大鼠随机分为sham组(假手术组)、DVT组和尿激酶(UK)组,DVT组和UK组大鼠制备深静脉血栓大鼠模型,术后1 d UK组给予尾静脉注入尿激酶20000 U/kg,每天1次,共注射14 d;sham组和DVT组给予相同容量的生理盐水。比较三组大鼠血栓的湿重和血栓湿重/长度的比值;用HE染色检测三组大鼠血栓组织的病理学变化;用酶联免疫吸附法(ELISA)检测三组大鼠血浆中炎症因子白介素8(IL-8)、肿瘤坏死因子α(TNF-α)的含量及可溶性血栓调节蛋白(sTM)的浓度;用实时荧光定量PCR(qRT-PCR)检测三组大鼠血栓调节蛋白(TM)mRNA的表达。结果DVT组出现明显血栓,其血栓湿重、血栓湿重/长度比值显著高于sham组(均P<0.05);尿激酶干预后的UK组大鼠血栓的湿重明显小于DVT组,且血栓湿重/长度比值明显小于DVT组(均P<0.05);和sham组比较,DVT组大鼠血栓明显机化,肉芽组织大量覆盖在组织周边,血管与管壁可见明显的粘连,静脉组织周围有大量的炎细胞浸润,瓣膜结构破坏明显;尿激酶干预后的UK组大鼠血栓组织得到明显的改善。和sham组相比,DVT组大鼠IL-8、TNF-α的含量及sTM的浓度均显著升高(均P<0.05),尿激酶干预后的UK组大鼠IL-8、TNF-α的含量及sTM的浓度显著低于DVT组(均P<0.05)。qRT-PCR结果显示DVT组TM mRNA表达明显高于sham组(P<0.05),与DVT组相比,UK组TM mRNA表达显著降低(P<0.05)。结论尿激酶对深静脉血栓体内的炎症因子有一定的抑制作用,同时抑制了血栓调节蛋白的表达。Objective To investigate the mechanism of urokinase on inflammatory substances and thrombomodulin(TM)in deep vein thrombosis(DVT)rats.Methods A rat model of deep vein thrombosis was established.Thirty rats were randomly divided into sham group,DVT group and UK(urokinase)group.The rat model of deep venous thrombosis was established in DVT group and UK group.One day after operation,urokinase(20000 U/kg)was injected into caudal vein in UK group once a day for 14 days;Sham group and DVT group were given the same volume of normal saline.The wet weight and the ratio of wet weight/length of thrombus were compared among the three groups;HE staining was used to detect the pathological changes of thrombus in the three groups;The plasma inflammatory factors interleukin-8(IL-8)and tumor necrosis factorα(TNF-α)were detected by enzyme-linked immunosorbent assay(ELISA).The mRNA expression of TM in the three groups was detected by real-time fluorescence quantitativepolymerase chain reaction(qRT-PCR).Results Compared with sham group,thrombosis was found in DVT group.The wet weight and wet weight/length ratio of thrombus in DVT group were significantly higher than those in sham group(P<0.05);After urokinase intervention,the wet weight of thrombus in UK group was significantly lower than that in DVT group,and the wet weight/length ratio of thrombus was also significantly lower than that in DVT group(P<0.05).Compared with sham group,DVT group had obvious thrombosis,granulation tissue covered around the tissue,obvious adhesion between blood vessels and tube wall,a large number of inflammatory cell infiltration around venous tissue and obvious destruction of valve structure;After urokinase intervention,the thrombus tissue of UK group was significantly improved.Compared with sham group,the concentration of IL-8,TNF-αand sTM in DVT group were significantly increased(P<0.05).After urokinase intervention,the IL-8,TNF-αand sTM concentration in UK group were significantly lower than those in DVT group(P<0.05).qRT-PCR results showed that

关 键 词：血栓调节蛋白 白细胞介素8 肿瘤坏死因子Α 深静脉血栓 尿激酶 

分 类 号：R543.6[医药卫生—心血管疾病]