大鼠血栓闭塞性脉管炎模型建立及靶向抑制NF-κB通路对TAO大鼠病变过程的影响  

Establishment of a rat model of thromboangiitis obliterans and the effect of targeted inhibition of NF-κB pathway on the pathological process of TAO model

作　　者：田野[1] 唐忠尉 张磊[1] 胡阳[2] 李新喜[1] Tian Ye;Tang Zhongwei;Zhang Lei;Hu Yang;Li Xinxi(Department of Vascular Surgery,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,Xinjiang,China;Department of Urology,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,Xinjiang,China)

机构地区：[1]新疆医科大学第一附属医院血管外科,新疆乌鲁木齐830000 [2]新疆医科大学第一附属医院泌尿外科,新疆乌鲁木齐830000

出　　处：《血管与腔内血管外科杂志》2021年第11期1291-1295,共5页Journal of Vascular and Endovascular Surgery

基　　金：新疆维吾尔自治区自然科学基金(2019D01C295)。

摘　　要：目的 探讨有效建立大鼠血栓闭塞性脉管炎(TAO)模型方式及靶向抑制核因子κB(NF-κB)通路对TAO大鼠病变过程的影响.方法 选用60只SPF级雄性Wistar大鼠,按照随机数字表法分为对照组、模型组、假手术组、NF-κB抑制7 d组、NF-κB抑制14 d组和NF-κB抑制21 d组.观察各组大鼠右后肢动脉血管病变情况.结果 TAO大鼠模型建立成功率为100%.模型组大鼠右后肢动脉血管腔内有血栓形成,血管周围有纤维组织增生.随着NF-κB抑制组的抑制时间增加,大鼠右后肢动脉出现炎性纤维组织增生和血管组织周围的炎性肉芽增生;对照组无明显变化.结论 本研究通过手术结合化学造模法制作了一种简单、可重复性高的大鼠TAO模型,并用该模型初步证实靶向抑制NF-κB通路可阻止TAO病情发展.Objective To investigate an effective approach to establish a rat model of thromboangiitis obliterans(TAO)and the effect of targeting inhibition of nuclear factor-κB(NF-κB)pathway on the pathological process of TAO rats.Method A total of 60 SPF male Wistar rats were selected and randomly divided into a control group,a model group,a sham operation group,an NF-κB inhibition group for 7 days,an NF-κB inhibition group for 14 days,and an NF-κB inhibition group for 21 days.The vascular lesions of the right hindlimb of the rats in each group were observed.Result The success rate of the TAO rat model establishment was 100%.In the model group,there was thrombosis in the arterial lumen of the right hindlimb and fibrous tissue proliferation around the blood vessels.With the prolonged inhibition time in the NF-κB inhibition group,inflammatory fibrous tissue hyperplasia and inflammatory granuloma hyperplasia around the vascular tissue appeared in the right hindlimb artery of the rats.However,there was no significant change in the control group.Conclusion The current study builds a simple and highly reproducible rat TAO model by combining surgery with chemical modeling,and the model was used to preliminarily confirm that targeting the NF-κB pathway could prevent TAO development.

关 键 词：血栓闭塞性脉管炎 动物模型 核因子ΚB 炎性反应 

分 类 号：R543[医药卫生—心血管疾病]