‘热研2号’柱花草对不同亲缘植物根系分泌物的生长响应  

Growth responses of Stylosanthes guianensis‘Reyan No.2’to root exudates from different relatives

作　　者：王俐媛 王坚[2] 赵元辰 许超 李雪枫 WANG Liyuan;WANG Jian;ZHAO Yuanchen;XU Zhao;LI Xuefeng(Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants,Ministry of Education/College of Forestry,Haikou 570228,Hainan,China;College of Animal Science and Technology,Hainan University,Haikou 570228,Hainan,China)

机构地区：[1]热带特色林木花卉遗传与种质创新教育部重点实验室/海南大学林学院,海南海口570228 [2]海南大学动物科技学院,海南海口570228

出　　处：《草业科学》2022年第2期309-317,共9页Pratacultural Science

基　　金：海南省自然科学基金(319MS016);海南省自然科学基金(317054);国家自然科学基金(31860682)。

摘　　要：植物间彼此存在交流行为,可以通过身份识别与亲缘植物进行相互合作与竞争。本试验旨在探究‘热研2号’柱花草(Stylosanthes guianensis‘Reyan No.2’)对不同亲缘植物根系分泌物的识别差异。将‘热研2号’柱花草分别在7种含有不同亲缘关系柱花草根系分泌物的营养液中培养,研究自我、种内近缘亲属、种间远缘亲属根系分泌物对‘热研2号’柱花草幼苗生长状况的影响。结果表明:与自我根系分泌物处理组相比,近亲处理组的侧根数量、总生物量、茎叶生物量和根生物量(鲜重)的平均值均显著增加(P<0.05),其中侧根数量和根生物量平均值增加250.00%和41.85%,而株高、根长和根冠比的平均值无显著差异(P>0.05);远亲处理组的根长、侧根数量、根生物量和根冠比的平均值均显著增加(P<0.05),而株高显著降低10.52%(P<0.05)。近亲和远亲处理组内各种/品种的具体响应结果存在差异。灰色关联分析表明,‘热研2号’柱花草对7种亲缘柱花草根系分泌物生长响应程度的强弱排序为‘有钩’>‘热研24号’>‘细茎’>‘西卡’>‘大叶’>‘热研5号’>‘热研21号’。由此说明亲缘识别是柱花草与其邻近植物竞争和相互作用的重要机制,在生产中将亲缘关系相近的柱花草品种混播(如‘热研2号’、‘热研5号’和‘热研21号’柱花草混播)是发展牧草混播新技术、增加单位面积饲草产量的可能途径。Plants communicate with each other and cooperate or compete with their relatives through identity recognition.The aim of this study was to clarify the identification differences of Stylosanthes guianensis‘Reyan No.2’on root exudates of different plant relatives.In this study,the effects of root exudates from S.guianensis‘Reyan No.2’itself and its close and distant relatives on seedling growth of this taxa were studied.This was undertaken by culturing them in seven nutrient solutions containing root exudates of different relatives of Stylosanthes.Results showed that the average values of lateral root number,total biomass,stem leaf biomass,and root biomass(fresh weight)in the close relative treatment group were significantly higher than those in the self root exudate treatment group(P<0.05).For the self-treatment group,the average values of lateral root number and root biomass increased by 250.00%and 41.85%,respectively.Meanwhile,the average values of plant height,root length,and root-shoot ratio were not significantly different(P>0.05).Compared with the self-treatment group,the average values of root length,lateral root number,root biomass,and root-shoot ratio in the distant treatment group significantly increased(P<0.05).In this group,plant height significantly decreased by 10.52%(P<0.05).There were differences in the specific response results for each species/variety in the close relative and distant relative treatment groups.Grey correlation analysis showed that the response of S.guianensis‘Reyan No.2’to the root exudates of seven Stylosanthes species was ranked as follows:S.hamata>S.guianensis‘Reyan No.24’>S.gracilis>S.scabra‘Seca’>S.grandifolia>S.guianensis‘Reyan No.5’>S.guianensis‘Reyan No.21’.It is suggested that kin recognition is an important mechanism for the competition and interaction between Stylosanthes and neighboring plants.In production,it is possible to develop new technologies for forage mixed sowing and increase forage yield per unit area by mixing Stylosanthes speci

关 键 词：亲缘识别 根系分泌物 生物量分配 根系生长 植物竞争 种内近亲 种间远亲 

分 类 号：S541.9[农业科学—作物学]