台湾拟金发藓二苯甲酮类化学成分及其细胞毒活性研究  

Benzophenones from endohydric moss Polytrichastrum formosum and their cytotoxic activities

作　　者：刘丽娜 张学文 侯芳洁 段绪红 赵建成[3] 陈玉玲[3] LIU Li-na;ZHANG Xue-wen;HOU Fang-jie;DUAN Xu-hong;ZHAO Jian-cheng;CHEN Yu-ling(Department of Pharmacy,Hengshui People′s Hospital,Hengshui 053000,China;Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,School of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;College of Life Science,Hebei Normal University,Shijiazhuang 050016,China)

机构地区：[1]衡水市人民医院药学部,河北衡水053000 [2]河北中医学院药学院,河北省中药炮制技术创新中心,河北石家庄050200 [3]河北师范大学生命科学学院,河北石家庄050016

出　　处：《中草药》2022年第3期667-670,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(31070184);河北中医学院博士科研基金资助项目(BSZ2021020);全国中药特色技术传承人才培训项目(T20194828003)。

摘　　要：目的对台湾拟金发藓Polytrichastrum formosum全草的化学成分及其细胞毒活性开展研究,为其进一步开发提供科学依据。方法采用硅胶、Sephadex LH-20凝胶、MCI-Gel树脂柱色谱、半制备型RP-HPLC等多种色谱技术进行分离纯化,运用波谱方法对所分离的化合物进行结构鉴定,采用MTT法测试化合物对人肝癌HepG2细胞和人胰腺癌PANC-1细胞的细胞毒活性。结果从台湾拟金发藓植物中分离得到4个化合物,分别鉴定为2′′-(1-苯甲酰基-2-羟基-4-苯氧基)-N-(2′′′-羟基-乙基)-乙酰胺(1)、(2-羟基-4-甲氧基-苯基)-苯基-甲酮(2)、(2,4-二甲氧基-苯基)-苯基-甲酮(3)、(2,4-二羟基-苯基)-苯基-甲酮(4);部分化合物显示出了一定的细胞毒活性。结论化合物1为新化合物,命名为金发藓酮B;化合物2~4为新天然产物,首次从金发藓科植物中分离得到;化合物1对人肝癌HepG2细胞具有较强的细胞毒活性,其半数抑制浓度(median inhibition concentration,IC_(50))值为38.14μmol/L。Objective To investigate the chemical constituents and cytotoxic activity of the whole plant of Polytrichastrum formosum,and provide scientific basis for its further development.Methods Various chromatographic techniques such as silica gel,Sephadex LH-20 gel,MCI-Gel resin column chromatography and semi-preparative RP-HPLC were used for separation and purification.Their structures were elucidated by spectroscopic data analyses.The cytotoxicity of compounds for HepG2 and PANC-1 cells line was also evaluated by using the MTT method.Results Four compounds were isolated and identified as 2′′-(1-benzoyl-2-hydroxy-4-phenoxy)-N-(2′′′-hydroxy-ethyl)-acetamide(1),(2-hydroxy-4-methoxy-phenyl)-phenyl-methanone(2),(2,4-dimethoxy-phenyl)-phenyl-methanone(3),and(2,4-dihydroxy-phenyl)-phenyl-methanone(4).Some compounds showed certain cytotoxic activity.Conclusion Compound 1 is a new compound named pogonatone B;Compounds 2—4 are new natural products,and isolated from Polytrichaceae species for the first time;Compound 1 also displays the high cytotoxicity on HepG2 cell with IC_(50) values of 38.14μmol/L.

关 键 词：金发藓科 台湾拟金发藓 二苯甲酮 金发藓酮B 细胞毒活性 

分 类 号：R284.1[医药卫生—中药学]