L02肝脂肪变性细胞膜快速筛选大黄调血脂活性成分  

Rapid screening of active components from Rhei Radix et Rhizoma for hypolipidemia by L02 hepatic steatosis cell membrane

作　　者：武晓玉 段文达 夏鹏飞 王玉霞 边惠琴 赵磊 WU Xiao-yu;DUAN Wen-da;XIA Peng-fei;WANG Yu-xia;BIAN Hui-qin;ZHAO Lei(Gansu University of Chinese Medicine,Lanzhou 730000,China;Key Laboratory of Chemistry and Quality for Traditional Chinese Medicine of Colleges of Gansu Province,Lanzhou 730000,China;Gansu Province Engineering Laboratory for Traditional Chinese Medicine Standardization Technology and Popularization,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学药学院,甘肃兰州730000 [2]甘肃省高校中(藏)药化学与质量研究省级重点实验室,甘肃兰州730000 [3]甘肃省道地药材质量标准化研究与推广工程实验室,甘肃兰州730000

出　　处：《中草药》2022年第3期735-742,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(82160457);甘肃省教育厅:青年博士基金项目(2021QB-079);甘肃中医药大学引进人才科研启动基金(2018YJRC-02);甘肃中医药大学科研发展基金项目(81660577)。

摘　　要：目的构建L02肝脂肪变性细胞膜固相色谱耦合脂肪变性模型,并将其应用于大黄调血脂活性成分的快速筛选。方法利用L02肝脂肪变性细胞膜作为固定相选择性地吸附大黄30%乙醇提取液中的活性成分,采用高效液相色谱(HPLC)测定吸附前后的化学成分;根据对照品的保留时间及紫外光谱信息,对比鉴定各亲和活性成分;并将筛选出的活性成分进一步作用于L02肝脂肪变性细胞模型,验证其调血脂作用。结果从大黄30%乙醇提取液中共筛选出11种活性成分,分别为芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和6种未知成分。芦荟大黄素等蒽醌苷元能够显著降低L02肝脂肪变性细胞中三酰甘油(triglyceride,TG)、总胆固醇(total cholesterol,TC)含量(P<0.01),且可减少L02肝脂肪变性细胞中的脂肪粒。结论建立的L02肝脂肪变性细胞膜固相色谱耦合脂肪变性模型可用于快速筛选中药复杂体系中的活性成分,为进一步深入探究大黄调血脂活性成分群奠定了基础。Objective To rapid screening the active components from Dahuang(Rhei Radix et Rhizoma)for hypolipidemia through L02 liver steatosis cell membrane solid phase chromatography coupled with steatosis model.Methods The active compounds of 30%ethanol extracts from Rhei Radix et Rhizoma were combined with L02 cell membrane solid phase chromatography.The solution was detected before and after treating with L02 cell membrane by HPLC.The active compounds from 30%ethanol extracts of Rhei Radix et Rhizoma were identified based on retention time and ultraviolet spectrum information of each chemical composition.The selected active ingredients were further applied to L02 hepatic steatosis cell model to verify the effect of hypolipidemia.Results A total of 11 compounds were detected from 30%ethanol extract of Rhei Radix et Rhizoma,and identified as aloe emodin,rhein,rheum emodin,chrysophanol,physcion and six unknown ingredients.Aloe emodin and other anthraquinone aglycones significantly reduced the contents of triglyceride(TG)and total cholesterol(TC)in L02 hepatic steatosis cell(P<0.01),and reduced the lipid granules in L02 hepatic steatosis cell.Conclusion The established L02 liver steatosis cell membrane solid phase chromatography coupled with steatosis model can be used for rapid screening of active components in the complex system of traditional Chinese medicine,as well as to lay the foundation for further study of the active components in hypolipidemia of Rhei Radix et Rhizoma.

关 键 词：大黄 L02肝脂肪变性细胞 细胞膜固相色谱 活性成分 芦荟大黄素 大黄酸 大黄素 大黄酚 大黄素甲醚 

分 类 号：R285.5[医药卫生—中药学]