花叶开唇兰与台湾银线兰的SSR标记技术鉴别  

Identification of Anoectochilus roxburghii and A.formosanus by SSR molecular markers

作　　者：王海阁 许文[1] 张勋[1] 许少华 徐伟[1] 林羽[1] 陈抒云[1] 黄泽豪[1] WANG Hai-ge;XU Wen;ZHANG Xun;XU Shao-hua;XU Wei;LIN Yu;CHEN Shu-yun;HUANG Ze-hao(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中草药》2022年第3期848-852,共5页Chinese Traditional and Herbal Drugs

基　　金：福建省自然科学基金资助项目(2017J01538);福建省科技厅高校产学合作项目(2019Y41010064);福建省科技厅引导性项目(2017Y0049,2018Y0052)。

摘　　要：目的利用SSR分子标记技术建立金线莲基原植物花叶开唇兰Anoectochilus roxburghii及其常见混淆品台湾银线兰A.formosanus的鉴别依据。方法根据花叶开唇兰转录组数据进行分析筛选SSR引物,通过DNA提取、PCR扩增以及引物多态性筛选等方法鉴别花叶开唇兰及台湾银线兰,从而鉴定药用金线莲真伪。结果基于花叶开唇兰转录组数据筛选出SSR多态性引物13对,UPGMA聚类法显示两者遗传相似系数变幅为0.38~1.00,在遗传相似系数0.38处,可将其完全分开。结论该研究构建了花叶开唇兰与台湾银线兰的分子标记鉴定体系和指纹图谱,为金线莲的商品鉴别及质量控制提供科学依据。Objective The purpose of this paper was to research the identification characteristics of Anoectochilus roxburghii and its adulterants of A.formosanus based on SSR molecular markers.Methods Firstly,SSR primers were selected according to the transcriptome data of A.roxburghii,and then the original plant of A.roxburghii and its adulterants of A.formosanus was identified by DNA extraction,PCR amplification and primer polymorphism screening,so as to identify the authenticity of Anoectochilus herba.Results By SSR molecular markers,13 pairs of SSR primers clustering analysis showed that the genetic similarity coefficient ranged from 0.38 to 1.00 so that A.roxburghii and A.formosanus could be separated at 0.38.Conclusion In this study,the molecular marker identification system and fingerprints of A.roxburghii and A.formosanus are established,which could provide scientific basis for commodity identification and quality control of Anoectochilus herba.

关 键 词：花叶开唇兰 台湾银线兰 SSR分子标记 真伪鉴别 指纹图谱 

分 类 号：R286.12[医药卫生—中药学]