不同膻味水平的龙陵黄山羊肝组织转录组SNP分析  

SNP analysis on liver transcriptome of Longling yellow goat with different odor levels

作　　者：马琳 杨明 王宣懿 叶绍辉[1] MA Lin;YANG Ming;WANG Xuan-yi;YE Shao-hui(College of Animal Science and Tecnology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物科学与技术学院,昆明650201

出　　处：《湖北农业科学》2022年第4期154-159,共6页Hubei Agricultural Sciences

基　　金：云南省肉羊产业技术体系项目(2017KJTX0016)。

摘　　要：为加强山羊肉类的质量,探究膻味相关差异基因,对3个品种共14只羊的总膻味值进行显著性差异检验发现,高膻味水平(总膻味值大于771.845)和低膻味水平(总膻味值低于771.845)龙陵黄山羊总膻味值差异显著(P<0.05)。通过对高膻味水平和低膻味水平的龙陵黄山羊肝组织进行转录组测序,将获得的数据进行SNP检测,并利用GO富集和KEGG富集分析SNP所在基因。结果表明,高膻味水平的龙陵黄山羊特有的SNP有411973个,低膻味水平的龙陵黄山羊特有的SNP有444638个,2者共有SNP 856611个;GO富集分析发现,SNP所在基因大量富集在生物进程和细胞组成中;KEGG富集分析发现,SNP所在基因显著富集在磷脂酰肌醇信号系统、补体和凝血级联、胰岛素抵抗、拼接体、自噬-动物、辅因子的生物合成、内质网中的蛋白质加工、胞吞作用、AMPK信号通路;对脂肪酸生物合成通路中的6个基因SNP分析发现,有7个SNP位于同义编码区,其中有2个SNP引起了氨基酸的改变。In order to enhance the quality of goat meat and explore the difference genes related to the odor,the total of 14 sheep of three breeds were tested for the significance difference of the total odor value.It was found that there was significant difference(P<0.05)between Longling yellow goat with high(total odor value greater than 771.845)and low(total odor value less than 771.845)lev⁃els.Transcriptome sequencing of liver tissues of Longling yellow goats with high and low odor levels was carried out,and SNP analysis was carried out on the obtained data,and SNP genes were analyzed by GO enrichment and KEGG enrichment.The results showed that there were 411973 SNPs peculiar to Longling yellow goats with high odor level and 444638 SNPs peculiar to Longling yellow goats with low odor level,and the common SNPs of two were 856611.GO enrichment analysis showed that SNP gene was enriched in biolog⁃ical process and cell composition.KEGG enrichment analysis showed that SNP gene was significantly enriched in phosphatidylinositol signaling system,complement and coagulation cascade,insulin resistance,splice,autophagy-animal,cofactor biosynthesis,protein processing in endoplasmic reticulum,endocytosis and AMPK signaling pathway.Analysis of SNPs of six genes in fatty acid biosynthe⁃sis pathway showed that seven SNPs were located in synonymous coding regions,and two SNPs caused amino acid changes.

关 键 词：龙陵黄山羊 肝脏 SNP GO富集 KEGG富集 

分 类 号：S826.89[农业科学—畜牧学]