提高番木瓜组培苗生根率的培养基配方优化  

Optimization of Rooting Medium for Tissue Culture of Papaya

作　　者：成镜[1] 李季[1] 戴雪梅[1] 徐正伟[1] 周权男[1] 黄天带[1] CHENG Jing;LI Ji;DAI Xuemei;XU Zhengwei;ZHOU Quannan;HUANG Tiandai(Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs,P.R.China/State Key Laboratory Incubation Base for Cultivation&Physiology of Tropical Crops/CATASRRI Engineering Center for Rapid Propagation of Tropical Woody Plants.,Haikou,Hainan 571101,China)

机构地区：[1]中国热带农业科学院橡胶研究所/农业农村部橡胶树生物学与遗传资源利用重点实验室/省部共建国家重点实验室培育基地-海南省热带作物栽培生理学重点实验室/热带林木种子种苗工程中心,海南海口571101

出　　处：《热带农业科学》2022年第2期60-64,共5页Chinese Journal of Tropical Agriculture

基　　金：海南天然橡胶产业集群(No.农产发[2020]2号)。

摘　　要：以TY-4番木瓜组培芽为试验材料,在以半强度Murashige和Skoog培养基为基本培养基,蛭石为支撑物的基础上,研究不同蔗糖浓度(0、5、20、40 g/L)、吲哚-3-丁酸浓度(2、4、6、8 mg/L)、间苯三酚浓度(0、10.0、14.9、19.9mg/L)对番木瓜生长状态和生根率及根系质量的影响。结果表明:将番木瓜组培芽在含蔗糖浓度为0,吲哚-3-丁酸(IBA)浓度为2 mg/L、间苯三酚浓度为0,并补充1/2强度的MS大量元素、MS微量元素、MS有机质,肌醇0.1 g/L、硫胺素0.4 mg/L和核黄素0.38 mg/L,支撑物为蛭石的培养基中培养时,体外芽最高生根率为95.2%,与最低的生根处理相比,高出38.1%,且茎基部形成愈伤组织较少。通过正交试验结果分析可知,番木瓜最佳的生根组合为:蔗糖浓度为0、吲哚-3-丁酸(IBA)浓度为2 mg/L、间苯三酚浓度为10.0 mg/L。本研究可为番木瓜体外繁殖生根技术提供理论参考。Tissue cultured shoots of papaya variety“TY-4”were cultured on rooting mediums with the half-strength Murashige and Skoog medium as basic medium and vermiculite as the support,supplemented with sucrose at different concentrations (0,5,20,40 g·L^(–1)),indole-3-butyric acid (IBA) at 2,4,6,8 mg·L^(–1),phloroglucinol at 0,10.0,14.9,19.9 mg·L^(–1)to observe the effects of the three factors,sucrose,IBA and phloroglucinol,on the growth,rooting rate and root quality.An orthogonal experiment was arranged to optimize the concentrations of the sucrose,IBA and phloroglucinol in the rooting medium.The results showed that when papaya tissue cultured shoots were cultured on the rooting medium including 1/2 strength MS with vermiculite as support,supplemented with macro-elements,trace elements,organic matter,0.1 g·L^(–1)inositol,0.4 mg·L^(–1)thiamine and 0.38 mg·L^(–1)riboflavin at the presence of 0 sucrose,2 mg·L^(–1)IBA,and 0 phloroglucinol,the rooting rate of the shoots in vitro was upto 95.2%,which was 38.1%higher than that of the treatment with the lowest rooting rate,under which less calli were formed at the base of the shoots.The orthogonal experiment showed that the optimal combination of the three factors in the papaya rooting medium is sucrose at the concentration of 0,IBA at 2 mg·L^(–1),and phloroglucinol at 10.0 mg·L^(–1).

关 键 词：番木瓜 蔗糖 吲哚-3-丁酸 间苯三酚 生根 根系质量 器官发生 蛭石 

分 类 号：S667.9[农业科学—果树学]