Antibacterial property of a gradient Cu-bearing titanium alloy by laser additive manufacturing  

作　　者：Dong-Yang Fan Zhe Yi Xu Feng Wen-Zhi Tian Da-Ke Xu A.M.Cristino Valentino Qiang Wang Hong-Chen Sun 

机构地区：[1]School and Hospital of Stomatology,China Medical University,Liaoning Provincial Key Laboratory of Oral Diseases,Shenyang 110002,China [2]Shenyang National Laboratory for Materials Science,Key Laboratory for Anisotropy and Texture of Materials(Ministry of Education),School of Materials Science and Engineering,Northeastern University,Shenyang 110819,China [3]Department of Electromechanical Engineering,University of Macao,Avenida da Universidade,Taipa,Macao,China

出　　处：《Rare Metals》2022年第2期580-593,共14页稀有金属（英文版）

基　　金：This study was financially supported by the National Natural Science Foundation of China(No.51871050);the Natural Science Foundation Project of Liaoning Province(Nos.2020-MS-150 and 2018225059);Shenyang Science and Technology Funded Project(No.RC190290).

摘　　要：Streptococcus mutans(S.mutans)is the most common cariogenic bacteria and causes caries by forming biofilms.A novel gradient Cu-bearing titanium alloy(TC4-5Cu/TC4)was manufactured using selective laser melting(SLM)technology for dental applications,which is anticipated to inhibit the formation of biofilm.In this study,the released concentration of copper ions in both minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)was tested in order to assess the antibacterial property of the alloy against planktonic S.mutans,and the antibacterial and antibiofilm efficiencies of TC4-5Cu/TC4 alloy against sessile S.mutans were evaluated via quantitative antibacterial tests and biofilm determination.Reverse transcription polymerase chain reaction(RT-PCR)was performed to analyze the expression of biofilm-related genes(gtfB,gtfC,gtfD,ftf and gbpB)and acid production-related gene(ldh).The results suggested that the MIC and MBC of Cu^(2+)were much higher than the release concentration of copper ions of the alloy,which was consistent with the lack of antibacterial effect against planktonic bacteria.On the contrary,TC4-5Cu/TC4 alloy exhibited significant bactericidal property against the sessile bacteria and efficient biofilm-restrained ability,and all genes detected in this research were down-regulated.The results indicated that the TC4-5Cu/TC4 alloy suppressed biofilm formation and the sessile bacterial viability by down-regulating biofilm-related genes.

关 键 词：COPPER Titanium alloys BIOFILM Antibacterial property Streptococcus mutans 

分 类 号：R318.08[医药卫生—生物医学工程] TG146.23[医药卫生—基础医学] TG665[一般工业技术—材料科学与工程]