典型多环芳烃对肺表面活性组分界面化学性质的影响  

Effects of typical polycyclic aromatic hydrocarbons on the interface chemical properties of pulmonary surfactant

作　　者：耿迎雪 曹妍 赵群[1] 李英杰[1] 师伟萌 刘丹 田森林[1] GENG Yingxue;CAO Yan;ZHAO Qun;LI Yingjie;SHI Weimeng;LIU Dan;TIAN Senlin(Faculty of Environmental Science and Engineering,Kunming University of Science and Technology,Kunming,650500,China;Faculty of Civil and Hydraulic Engineering,Xichang University,Xichang,615013,China)

机构地区：[1]昆明理工大学环境科学与工程学院,昆明650500 [2]西昌学院土木与水利工程学院,西昌615013

出　　处：《环境化学》2022年第2期439-449,共11页Environmental Chemistry

基　　金：国家自然科学基金(21777064,41761072)资助。

摘　　要：多环芳烃由于其高亲脂性,随呼吸到达肺部可在深肺区沉积,并与覆盖于肺泡内衬层的肺表面活性物质接触并反应,进而影响肺表面活性物质的界面活性.选取典型多环芳烃苯并[a]蒽为研究对象,研究其对气-液界面处DPPC单分子膜界面化学性质的影响,以此来探究多环芳烃对肺功能的毒性行为.采用Langmuir膜天平获得DPPC单分子膜的压缩-扩张循环曲线;通过弹性模量分析苯并[a]蒽对单分子膜弹性性能的影响;借助布儒斯特角显微镜,对DPPC单分子膜的微观形貌进行原位观测;通过激光共聚焦显微拉曼光谱分析苯并[a]蒽对DPPC分子内部结构的影响.结果表明,苯并[a]蒽可显著影响磷脂单层的相行为,表面压-面积等温线向高的分子面积区域移动,在压缩阶段,膜的刚性及稳定性减弱.布儒斯特角原位实验表明,苯并[a]蒽以嵌入的形式存在于DPPC单层之间,DPPC单分子膜分布不均,靠近苯并[a]蒽区域较密集,远离区域排列疏松、出现相的分离.拉曼光谱进一步说明,苯并[a]蒽影响DPPC分子疏水烷基链C—C及C—H的伸缩振动,造成脂链有序构象增多、膜的流动性减弱,进而影响呼吸相关功能的发挥.Polycyclic aromatic hydrocarbons(PAHs)are highly hydrophobic,thus on first contacting with pulmonary surfactant,they will accumulate in the monolayer,leading to pulmonary surfactant malfunctioning and respiratory disorders.1,2-dipalmitoyl-sn-glycero-3-phospho-choline(DPPC)is the main active component of pulmonary surfactant,and it is usually used as the mimic and substitute of pulmonary surfactant.The effect of benz[a]anthracene on the interface chemical properties of DPPC monolayers at the gas-liquid interface was investigated.The compression expansion cycle curves of DPPC monolayers were obtained by Langmuir membrane balance;the effect of benz[a]anthracene on the elastic properties of DPPC monolayers was analyzed by elastic modulus;the micro morphology of DPPC monolayers was observed by Brewster angle microscope;the internal structure of DPPC was analyzed by laser confocal micro Raman spectroscopy.The results show that the presence of benz[a]anthracene can cause the surface pressure area isotherm to move to the region with high molecular area,reduce the aggregation degree of DPPC monolayers,and loose arrangement of individual regions,resulting in phase separation.Raman spectra further showed that benz[a]anthracene affected the stretching vibration of hydrophobic alkyl chain C—C and C—H of DPPC molecule,resulting in the increase of ordered conformation of lipid chain and the decrease of membrane fluidity,which further affected the respiratory related function.

关 键 词：多环芳烃 1 2-二棕榈酰-sn-甘油-3-磷酸胆碱 肺表面活性剂 界面化学性质 微观形貌 

分 类 号：R114[医药卫生—卫生毒理学] X513[医药卫生—公共卫生与预防医学]