UV-B处理对大豆异黄酮合成影响及GmUVR8基因克隆与表达分析  

Analysis of Isoflavone Synthesis Influenced by UV-B Treatment and Cloning of GmUVR8 Gene in Soybean

作　　者：张艺 于存浩 马晓红[1] 姚陆铭[1] 武天龙[1] 王彪[1] ZHANG Yi;YU Cun-hao;MA Xiao-hong;YAO Lu-ming;WU Tian-long;WANG Biao(School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China;College of Agriculture,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]东北农业大学农学院,黑龙江哈尔滨150030

出　　处：《大豆科学》2022年第1期28-35,共8页Soybean Science

基　　金：国家自然科学基金(31871645)。

摘　　要：为了探究UV-B辐射对大豆异黄酮合成调控的分子机理,本研究以UV-B辐射处理大豆V1期幼苗,采用HPLC和定量PCR方法分别测定处理前后各部位异黄酮含量和基因的表达,并克隆出GmUVR8光受体基因。发现UV-B辐射处理大豆V1期幼苗8 h后,叶中总异黄酮含量提高1.3倍,大豆苷元和染料木素分别提高15.8和16.5倍。大豆根、茎和叶中CHS和IFS基因对UV-B反应的时间和强度存在明显差异,CHS11在处理2 h后表达量增加38.4倍,IFS1和IFS2基因的最高表达量分别比处理前增加4.7和18.3倍。克隆出了大豆中编码UVR8光受体的GmUVR8a、GmUVR8b和GmUVR8c基因,其氨基酸序列与拟南芥AtUVR8的同源度为74%;GmUVR8a、GmUVR8b、GmUVR8c基因在叶中表达存在显著差异,以GmUVR8b表达量最高。结果表明在大豆中UV-B辐射可能通过多种UVR8光受体调控苯基丙酸类途径关键酶基因的表达,进而影响异黄酮的合成。In order to explore molecular mechanism of soybean isoflavone synthesis influenced by UV-B radiation,isoflavone accumulation and gene expression levels in different parts of V1 stage,seedlings under UV-B treatment were investigated by HPLC and quantitative real-time PCR respectively,and genes encoding UV RESISTANCE LOCUS 8(UVR8)photoreceptor were also cloned.The accumulation of total isoflavones in leaves of soybean V1 stage was increased by 1.3 times under 8 h UV-B radiation,and daidzein,genistein improved by 15.8 and 16.5 times respectively.The transcripts of CHS and IFS exhibited obvious difference response to time after UV-B stress,and CHS11 was increased 38.4 times after 2 h treatment,and the highest expression levels of IFS1 and IFS2 increased by 4.7 and 18.3 times respectively.The coding sequences of UVR8 photoreceptor GmUVR8 a,GmUVR8 b and GmUVR8 c were cloned from soybean,the encoding protein had 74%homology in amino acids with AtUVR8,their expression levels in leaves showed significant difference,and GmUVR8 b displayed the highest transcripts among those genes.The results indicated that UV-B radiation might regulate synthesis of soybean isoflavones through multiple UVR8 light receptors which affect the expression of key enzymes in phenylpropanoid pathway.

关 键 词：大豆 UV-B UVR8光受体 异黄酮 基因表达 基因克隆 

分 类 号：S565.1[农业科学—作物学]