百蕊草药材中5种黄酮类化学成分的含量测定  

Determination of Five Kinds of Flavonoids in the Medicinal Herbs of Thesium chinensis

作　　者：郑玲玲 纪瑞锋 韩红亮 罗旖璐 罗嘉仪 管悦琴 姚其盛 郑卫兵 陈美兰[1] 周修腾[1] ZHENG Lingling;JI Ruifeng;HAN Hongliang;LUO Yilu;LUO Jiayi;GUAN Yueqin;YAO Qisheng;ZHENG Weibing;CHEN Meilan;ZHOU Xiuteng(China Academy of Chinese Medical Sciences,National Resource Center for Chinese Material Medical/State Key Laboratory Breeding Base of Dao-di Herbs,Beijing 100700;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006;Anhui Jiuhua Huayuan Pharmaceutical Co.,Ltd.,Chuzhou,Anhui 239000)

机构地区：[1]中国中医科学院中药资源中心/道地药材国家重点实验室培育基地,北京100700 [2]广东药科大学中药学院,广州510006 [3]安徽九华华源药业有限公司,安徽滁州239000

出　　处：《中国农学通报》2022年第5期30-36,共7页Chinese Agricultural Science Bulletin

基　　金：中央本级重大增减支项目“名贵中药资源可持续利用能力建设”(2060302),国家自然科学基金青年科学基金项目(81803658)。

摘　　要：为了揭示野生与人工栽培百蕊草5种黄酮类化学成分含量的差异,本研究采用液相色谱分析方法测定百蕊草中百蕊草素Ⅰ、山奈酚-3-O-芸香糖苷、紫云英苷、山奈酚-7-O-葡萄糖苷、芹菜素-7-O-β-D吡喃葡萄糖苷的含量。结果表明百蕊草素Ⅰ、山奈酚-3-O-芸香糖苷、紫云英苷、山奈酚-7-O-葡萄糖苷、芹菜素-7-O-β-D-吡喃葡萄糖苷线性范围分别为10~1000(0.9995)、1~200(0.9992)、1~200(0.9995)、1~200(0.9994)、10~2000µg/mL(0.9998);精密度、稳定性及重复性试验的RSD为0.17%~3%;平均加样回收率为91.99%~107.40%,RSD均小于3.24%。与野生百蕊草相比,人工栽培百蕊草中百蕊草素Ⅰ、紫云英苷、山奈酚-7-O-葡萄糖苷及芹菜素-7-O-β-D-吡喃葡萄糖苷含量均显著(P<0.05)高于野生百蕊草,而山奈酚3-O-芸香糖苷含量有高于野生百蕊草趋势,但是并无显著性差异。本研究表明该方法操作重复性好、准确、简便,可同时测定野生与人工种植百蕊草中5个黄酮类化学成分的含量,建议推广使用。To reveal the differences in the contents of five kinds of flavonoids between wild and cultivated Thesium chinensis,the contents of Kaempferol-3-O-rhamnose(1-2)glucoside,Kaempferol-3-O-rutinoside,Astragaline,Kaempferol-7-glucoside and Apigenin-7-O-β-D-glucopyranoside in Thesium chinensis were determined by Ultra-High Performance Liquid Phase.The results showed that the linear ranges of Kaempferol3-O-rhamnose(1-2)glucoside,Kaempferol-3-O-rutinoside,Astragaline,Kaempferol-7-glucoside and Apigenin-7-O-β-D-glucopyranoside were 10-1000(0.9995),1-200(0.9992),1-200(0.9995),1-200(0.9994),10-2000 g/mL(0.9998),respectively;RSD of precision,stability and reproducibility tests was 0.17%~3%.The average recoveries of the five kinds of flavonoids were 91.99%~107.40%with RSD less than 3.24%.Compared with that of the wild Thesium chinensis,the contents of Kaempferol-3-O-rhamnose(1-2)glucoside,Astragaline,Kaempferol-7-glucoside and Apigenin-7-O-β-D-glucopyranoside in cultivated Thesium chinensis were significantly higher(P<0.05),and the content of Kaempferol-3-O-rutinoside tended to be higher but showed no significant difference.This study shows that the method has good repeatability,accuracy and simplicity.It can determine the contents of the five kinds of flavonoids in wild and cultivated Thesium chinensis at the same time,and is recommended to be popularized.

关 键 词：百蕊草 黄酮类化学成分 超高效液相 野生与栽培 正交试验 

分 类 号：S567.23[农业科学—中草药栽培]