饲喂柞蚕免疫蛹粉对红鳍东方鲀生长、免疫和抗病力的影响  

Effects of Dietary Immunity Induced Pupae Powder of Antheraea pernyi on Growth, Non-Specific Immunity and Disease Resistance of Tiger Puffer Takifugu rubripes

作　　者：孙永欣 马淑慧 王庆志[1] 任同军 都兴范 李树英 SUN Yongxin;MA Shuhui;WANG Qingzhi;REN Tongjun;DU Xingfan;LI Shuying(Dalian Key Laboratory of Genetic Resources for Marine Shellfish,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China;College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China)

机构地区：[1]辽宁省海洋水产科学研究院,大连市海产贝类种质资源创新利用重点实验室,辽宁大连116023 [2]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《水产科学》2022年第2期236-242,共7页Fisheries Science

基　　金：国家蚕桑产业技术体系项目(CARS-18-02A)。

摘　　要：利用柞蚕蛹体外诱导产生抗菌活性物质的特性,制备柞蚕免疫蛹粉作为饲料添加剂,考察其对(58.52±2.07) g红鳍东方鲀幼鱼生长、免疫力和抗病力的影响。试验共分5组:基础饲料组作为空白组,基础饲料中分别添加0.5%、1.0%和2.0%免疫蛹粉作为试验组,另设定1.0%普通蛹粉作为对照组,试验周期共30 d。试验结果表明,随着饲料中免疫蛹粉添加量增加,3个添加组红鳍东方鲀质量增加率也随之提高,与空白组相比均差异显著(P<0.05),其中1.0%和2.0%组显著高于空白组和对照组(P<0.05);随着饲料中免疫蛹粉添加量增加,其对红鳍东方鲀血清免疫指标影响各异,其中0.5%免疫蛹粉组酸性磷酸酶、超氧化物歧化酶和过氧化氢酶活性显著高于空白组和对照组(P<0.05),1.0%组碱性磷酸酶和过氧化氢酶活性显著高于空白组和对照组(P<0.05),2.0%组过氧化氢酶活性显著高于空白组和对照组(P<0.05)。体内注射致病菌考察免疫保护率,结果表明,空白组的7 d累计死亡率为100%,红鳍东方鲀摄食免疫蛹粉后明显提高免疫保护率,其中0.5%组最高,达50%。综上,饲料添加0.5%免疫蛹粉对提高红鳍东方鲀质量增加率、免疫力和抗病力的效果最好,可用于实际生产养殖。Immunity induced pupae powder(IPP) of Antheraea pernyi was supplemented as feed additive to investigate its effects on the growth, immunity and disease resistance of the juvenile tiger buffer Takifugu rubripes with body weight of(58.52±2.07) g. There were five groups in triplication including basic feed group as the blank group, 0.5%, 1.0% and 2.0% dose of IPP were added to the basic feed as the test group, and 1.0% pupa powder was added to the basic feed as the control group. The supplementary feeding experimental lasted for 30 days. The results showed that the weight gain rate of the fish was significantly increased with the increase in the amount of IPP in the feed compared with the blank group(P<0.05). Furthermore, the fish fed diets containing 1.0% and 2.0% IPP had significantly higher weight gain rate than the fish in the blank group and the control group(P<0.05). The effects of IPP on the serum immune index were found different in various aspects did. The activities of acid phosphatase(ACP), superoxide dismutase(SOD) and catalase(CAT) in 0.5% IPP group, alkine phosphatase(AKP) and CAT in 1.0% IPP group, and CAT in 2.0% IPP group were significantly higher than those in blank group and control group(P<0.05). The immunoprotective effect was analysed using the pathogenicity challenge test and the cumulative mortality rate in the blank group was 100% after 7 days, and the immunoprotective rate was increased obviously, with the maximal immunoprotective rate of 50% in 0.5% IPP group. Above all, the supplement of 0.5% IPP in diet led to effectively increase weight gain rate, immunity and disease resistance, which can be used in fish production.

关 键 词：红鳍东方鲀 免疫蛹粉 生长 免疫 

分 类 号：S965.225[农业科学—水产养殖]