恩诺沙星对西伯利亚鲟血清和肝脏中转氨酶活性的影响  

Effects of Enrofloxacin on Transaminase Activity in Serum and Liver of Siberian Sturgeon Acipenser baeri

作　　者：刘盼[1] 杨慕[1] 贾成霞[1] 张清靖[1] 曲疆奇[1] LIU Pan;YANG Mu;JIA Chengxia;ZHANG Qingjing;QU Jiangqi(Fishery Resources and Environment Observation and Experimental Station in Northern China,Ministry of Agriculture and Rural Affairs,Beijing Key Laboratory of Fishery Biotechnology,Beijing Fisheries Research Institute,Beijing 100068,China)

机构地区：[1]北京市水产科学研究所渔业生物技术北京市重点实验室,农业农村部北方地区渔业资源环境观测试验站,北京100068

出　　处：《水产科学》2022年第2期273-279,共7页Fisheries Science

基　　金：现代农业产业技术体系北京市鲟鱼鲑鳟鱼创新团队项目(BAIC08)。

摘　　要：分别在15℃和26℃下将恩诺沙星以10 mg/kg质量浓度对西伯利亚鲟口灌给药,测定给药后0.125、0.25、0.5、1.0、1.5、2、4、6、8、12、18、24、36、48、60、72、96 h和120 h血清及肝脏组织中谷草转氨酶和谷丙转氨酶活性随时间的变化趋势。试验结果显示,两个温度下给药后,各时间点谷草转氨酶、谷丙转氨酶活性均有所升高。15℃下,在6~24 h,血清中谷草转氨酶活性显著高于肝脏中谷草转氨酶;36~120 h(除72 h外)肝脏中谷草转氨酶活性又显著高于血清中谷草转氨酶;0.25~4 h和36~120 h肝脏内谷丙转氨酶活性显著高于血清中谷丙转氨酶。26℃下,6~120 h血清中谷草转氨酶活性显著高于肝脏中谷草转氨酶;在0~0.5 h,肝脏中谷丙转氨酶活性显著高于血清中谷丙转氨酶,而1.5~120 h血清中谷丙转氨酶活性则显著高于肝脏中谷丙转氨酶。试验结果显示,口灌恩诺沙星后,血清和肝脏中转氨酶类(谷草转氨酶、谷丙转氨酶)活性均表现为逐渐升高至一定水平后缓慢下降和维持平稳,但26℃下18~36 h时间段内血清中谷草转氨酶和谷丙转氨酶活性显著高于肝脏中的活性,说明肝细胞已在一定程度上受损。试验结果表明,在适宜温度下,以10 mg/kg剂量给药,恩诺沙星对西伯利亚鲟血清和肝脏无明显毒性作用。该试验结果将为恩诺沙星在西伯利亚鲟养殖中的合理使用提供理论基础。Enrofloxacin was orally administered to Siberian sturgeon Acipenser baeri at 15 ℃ and 26 ℃, respectively. The changes in glutamic-oxalacetic transaminase(GOT) and glumtaic-pyruvic transaminase(GPT) activities were measured with time in serum and liver tissues at 0.125 h, 0.25 h, 0.5 h, 1.0 h, 1.5 h, 2 h, 4 h, 6 h, 8 h, 12 h, 18 h, 24 h, 36 h, 48 h, 60 h, 72 h, 96 h and 120 h after administration. The results showed that the activities of GOT and GPT were increased at two temperatures. At 15 ℃, the activity of GOT was significantly higher in serum than that in liver within 6—24 h;at 36—120 h(except 72 h), the activity of GOT was significantly higher in liver than that in serum. GPT activity was significantly higher in liver than that in serum in 0.25—4 h, and was significantly higher in liver than that in serum in 36—120 h. At 26 ℃, the activity of GOT was significantly higher in serum than that in liver within 6—120 h;at 0—0.5 h, the activity of GPT was significantly higher in serum than that in liver, while from 1.5—120 h, the activity of GPT was significantly higher in liver than that in serum. It was found that after oral administration of enrofloxacin, the activities of transaminases(GOT and GPT) in serum and liver were gradually increased to a certain extent, then slowly decreased and remained stable. However, the content of GOT was significantly higher in serum in 18—36 h at 26 ℃ than that in liver, indicating that liver cells had been damaged to a certain extent. The enrofloxacin had no significant toxic effect on the serum and liver of Siberian sturgeon at the appropriate temperature and 10 mg/kg body weight. The findings provide theoretical basis for the rational use of enrofloxacin in the breeding of Siberian sturgeon.

关 键 词：恩诺沙星 西伯利亚鲟 谷草转氨酶 谷丙转氨酶 

分 类 号：S948[农业科学—水产养殖]