高温应激下维生素E对红耳龟免疫指标及消化酶的影响  

Effect of Vitamin E on Immune Index and Digestive Enzyme Activity of Red-Eared Turtle Trachemys scripta elegans under High Temperature Stress

作　　者：雷金洪 刘天天 张广武 傅丽容[1] 史海涛[1] LEI Jinhong;LIU Tiantian;ZHANG Guangwu;FU Lirong;SHI Haitao(Key Laboratory for Ecology of Tropical Island,Ministry of Education,College of Life Sciences,Hainan Normal University,Haikou 571158,China)

机构地区：[1]海南师范大学生命科学学院,热带岛屿生态学教育部重点实验室,海南海口571158

出　　处：《水产科学》2022年第2期311-315,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(31772486)。

摘　　要：为探讨高温胁迫下维生素E对红耳龟幼龟血清免疫指标和肠道消化酶活性变化的影响,选取体质量(108.42±19.56) g健康红耳龟幼龟40只,随机分成4组,每组2个平行,每个平行5只龟,每组饲料中维生素E的添加量分别为0(对照组)、150、250、350 mg/kg,置于(34±0.5)℃高温环境中,饲养30 d,取幼龟血清和肠道组织,检测血清抗氧化酶和肠道消化酶的活性。试验结果表明,维生素E 250 mg/kg试验组超氧化物歧化酶和3个试验组过氧化氢酶活性均极显著高于对照组(P<0.01),其中250 mg/kg的添加量效果尤为显著,试验组丙二醛含量均低于对照组,差异极显著(P<0.01)。维生素E对肠道淀粉酶活性无显著影响(P>0.05),但极显著或显著影响蛋白酶和脂肪酶活性(P<0.01或P<0.05),试验组脂肪酶活性显著低于对照组(P<0.05),250 mg/kg试验组活性最低;蛋白酶的活性变化相反,试验组显著高于对照组(P<0.05),250 mg/kg试验组活性最高。综上所述,高温胁迫环境下,日粮中添加维生素E,可以显著提高红耳龟幼龟的抗应激能力,在一定程度上提高肉食性喂养的红耳龟幼龟的蛋白消化能力,以250 mg/kg维生素E添加量为宜。To investigate the effect of vitamin E level on blood immune index and digestive enzyme activity of red-eared turtle Trachemys scripta elegans under high temperature stress, forty healthy juvenile red-eared turtles with initial body mass of(108.42±19.56) g were randomly divided into 4 groups at(34±0.5) ℃ for 30-day feeding trial. There were two parallels in each group, and five turtles in each parallel. The turtles in each group were fed the diets containing vitamin E at a dose of 0(control group), 150, 250 and 350 mg/kg, respectively. The activities of serum antioxidant enzymes and intestinal digestive enzymes in serum and intestinal tissues of juvenile turtles were detected respectively. The results showed that the activities of superoxide dismutase in the 250 mg/kg group and catalase in the 3 treatment groups were significantly higher than those in the control group, especially in the 250 mg/kg group. The contents of malondialdehyde in the treatment groups were significantly lower than that in the control group(P<0.01), vitamin E did not lead to affect on intestinal amylase activity significantly(P>0.05), but very significant/significant effect on protease and lipase activities(P<0.01, P<0.05).The lipase activity of the experimental groups was significantly lower than that in control group, with the minimal activity in the 250 mg/kg group. On the contrary, the activity of protease in treatment groups was significantly higher than that in the control group, with the maximal activity in 250 mg/kg group. To sum up, the addition of vitamin E in diet led to significantly improve the anti-stress ability of juevenile red-eared turtle under high temperature stress environment, and improve the protein digestibility within limits of juevenile red-eared turtle fed meat. The addition of vitamin E in 250 mg/kg was the optimal for diet of red-eared turtle.

关 键 词：红耳龟 维生素E 高温应激 抗氧化酶 消化酶 

分 类 号：S966.5[农业科学—水产养殖]